PART VI 
APPENDIX 
CHAPTER XXI 
LABORATORY WORK 
Laboratory work should, so far as possible, accompany the study of the text. 
It is impossible to make a series of experiments that will closely follow the order 
of topics in the text, since a large amount of preliminary work has to be done in 
making media before actual study of bacteria begins. The order of experiments 
below given will be found a convenient one to follow, but may be modified to suit 
convenience. 
APPARATUS NEEDED 
Steam sterilizer. 
Autoclave. 
Hot air sterilizer—A common gas oven used for cooking will do. 
Stew-pan for cooking media. 
Flasks.—Liter and half-liter. 
Test-tubes, heavy—Board of Health pattern. 
Petri dishes—four inches in diameter. 
Pipettes—z c.c. and 2c.c. Some larger ones are also convenient. Each of the 
smaller ones should have a glass tube holder (Fig. 56) or there should be a metal 
box with cover to hold fifty or more pipettes at once. 
Capillary pipettes—graduated to deliver o.or c.c. 
Wire baskets to hold test-tubes. 
Test-tube racks, or common tumblers with a little cotton in the bottom to hold 
test-tubes. 
Beakers. 
Evaporating dishes. 
Fermentation tubes. 
Burette holder with four burettes. 
Evaporating dishes. 
Measuring cylinders—rz liter and 100 c.c. 
331 
