SMILLIE’S ANAEROBIC METHOD 21 
the circuit. In the coil of the fine nichrome wire is placed a small mass 
of platinized asbestos #. The apparatus is placed in a package and 
autoclaved.” Smillie has applied the same method to the culture of 
large amounts of bacteria in Blake bottles. 
This method applied to the culture of bacteria in anaerobic jars is 
as follows: 
Smillie experienced some trouble in getting jars which would stand 
up under great vacuum. He finally adopted the ordinary specimen 
Connecting rubber tube. 
Glass Bulb containing platinized asbestos. 
2mm. perforations, 
ey 
Fie. 21.— Detail of Platinized Asbestos Bulb for Anaerobic Jar According to Smillie’s 
Method. 
jar about 30 em. high and an inside diameter of 12.5 em. In the cover 
of this were ground two holes into which were fitted No. 4 one-hole 
rubber stoppers, carrying a glass ‘‘ angle” stop-cock. A rubber tube 
extending to the bottom of the jar was attached to one of these. To 
the other stop-cock, a glass bulb with perforations is attached by means 
