22 BACTERIOLOGICAL APPARATUS 
of rubber tubing. This glass bulb is filled with platinized asbestos. 
The cultures are placed in a glass tumbler which is put into the Jar. 
Fig. 21 taken from Smillie’s original publication will give the details 
of the asbestos bulb. 
EXCLUSION OF OxYGEN 
Beattie’s Method. Ordinary test tubes containing plain or car- 
bohydrate broths are used. Melted sterilized vaseline is poured into 
the tube until a plug about 4 in. long is formed. The broth and vase- 
line are then boiled for twenty to thirty minutes in order to expel any 
dissolved air and allowed to cool. These tubes may be kept for an 
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Fig. 22.—Types of Novy Jars. 
indefinite time and are air tight. When they are to be inoculated they 
may be melted in a water bath at 55° C. The sample may be intro- 
duced by means of a sterile pipette. The vaseline may then be heated 
over a flame to reform the solid plug. The tube may then be placed 
in the incubator. In order to remove any portion of the culture, a 
sterile sealed pipette (capillary) is thrust through the plug. This may 
be broken by forcing the end against the bottom of the tube after which 
any portion of the culture may be removed. By melting the vaseline 
plug again the culture tube is sealed for further incubation. 
Novy’s Method. The apparatus used in the application of this 
method consists of a large glass jar with a removable top (Fig. 22). 
The culture may be grown away from oxygen by using an inert gas 
vacuum or alkalin pyrogallol. The jar is constructed with a glass stop- 
cock which will allow the removal of air with a vacuum pump. This 
