AGAR MEDIA 49 
are free from many of the objections incident to plates made from a 
lesser per cent of agar. The results obtained were approximately equiv- 
alent to Endo’s medium agar. 
Trypagar (Gordon etal). Take a measured quantity of the trypsinized 
broth, add 2 per cent of agar fiber, and 0.125 gm. of calcium chloride 
per liter. Autoclave at 118° C. for three-fourths hour to dissolve the 
agar. Mix together in a saucepan; titrate with N/10 KOH to give an 
absolutely neutral reaction. Cool to 60° C., add white of two eggs 
beaten up with the crushed shells, autoclave again at 118° C. for seventy- 
five minutes (or in the steamer for two hours). Filter, add to the filtrate 
5 per cent of the sterile pea extract, and sterilize in the ordinary way. 
Neutral Red Bile Salt Agar (Savage, 1901). 
Sodium taurocholate..2.....0..0.0.0.. 0000008 5 gms. 
Witte’s peptone... 2... 0... cee ee eee 20 gms. 
Distilled water.................. re 1000 c.c. 
AQAP. eect enen ene es 20 gms. 
After filtration 10 gms. lactose and 5 c.c. of a 1 per cent solution 
of neutral red are added. 
Glucose Formate Agar (Kitasato). 
Nutrient agar... 6... ee ees -... 1000 c.c. 
Glucose... 0... cee enn eee 20 gms. 
Sodium formate... ... 0... cece eee ees 4 gms. 
Tube and sterilize. 
Soil Extract Agar (Conn, 1914). 
AQOD.. cece eee nets 15 gms 
Dextrose... cece eee eee eee 1 gm 
Soil extract... 02... cece eee teres 100 c.c 
0 ar 900 c.c 
Synthetic Agar. 
Dextrose. 0... cece eee et eee teens 10 gms. 
MgSO04... 2-0. ccc cee ete eee teens 0.2 gms. 
KoHPO4. 2... ccc eee tence ees 0.5 gm 
Peptone...... 0.0.02 cece eee eect eee 0.05 gm 
AGAT.. eee ees 20 gms 
