SPECIAL AGAR MEDIA 
Sabouraud’s Agar for Yeasts (after Anderson, 1917). 
AZAl. occ cece tenet eee ees 20 gms. 
Peptone.... 0.0... cece eee ee eens 10 gms. 
G]UCOSe. 2. eee ete ee 40 gms. 
Water 2... ccc ccc cece cece eee eees 1000 c.c. 
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Mix these ingredients in the usual way and adjust the reaction to 
+2 per cent acid. Without further heating the sugar is added and the 
medium tubed in previously sterilized tubes. Anderson advises care 
in sterilizing since the high acidity of this medium may prevent solidi- 
fication of the agar. 
Agar Gelatin Medium (North 1909). 
Lean chopped beef or veal.................. 500 gms 
ABAr.. cee eens 10 gms 
Gelatin. 2... ec cee eens 20 gms 
Peptone.... 0... ee eee eee eee eens 20 gms 
Nal... ccc ccc cee e ete eeenee 5 gms. 
Distilled water... .......... 0. eee e eee ee eee 1000 c.c. 
Adjust neutral to phenolphthalein. North states that this medium 
is excellent for streptococci, pneumococci and diphtheria bacilli because 
it is soft and moist and can be used at 37° C. It is claimed to be of 
special value in keeping stock cultures. 
Hesse Medium. 
Fk: a 5 gms 
Peptone........ 0... eee eee ee ee eee 10 gms. 
Liebig’s beef extract.......... 0.0.0.0. eee 5 gms. 
Sodium chloride...............-..-220 eee: 8.5 gms. 
Distilled water... 0.0.0... 0.0. c eee eee ee eee 1000 c.e. 
Agar is dissolved in 500 c.c. H2O. Other ingredients are dissolved 
in 500 c.c. HeO. Solutions are mixed, filtered and tubed. 
Hiss Plating Medium (1897). 
AQAP. cece cee cee eee cence eens 15 gms 
Gelatin... 0... ce cc cee ee eens 15 gms, 
Liebig’s meat extract... ... 2.0.0... ee eee eee 5 gms 
NaCl............. eee cee eee eee ee eee 5 gms. 
Dextrose... 0... cece eect ee ene eee 10 gms. 
Distilled water..............0ccceeceeeeeee 1000 c.e. 
