64. MEDIA AND THEIR PREPARATION 
Capaldi’s Medium. 
PeptOne.. 2.6 c eee ccc cece ee eee eens 20 gms. 
Gelatin. 0... ccc cece eens 10 gms. 
Fg 5 20 gms 
Dextrose or mannit... 0.0.0.0... 02 cee eee eee 10 gms 
Sodium chloride, NaCl................2005. 5 gms. 
Potassium chloride, KCl.................4. 5 gms. 
Distilled water... .....0.. 00. ccc ene eee es 1000 c.c. 
Frankel’s Solution. 
Sodium chloride, NaCl...................5. 5 gms. 
Monocalcium phosphate, Ca(H2PO4)e....... 2 gms. 
Ammonium lactate, CHs—CHOH—CONH)b.. 6 gms. 
. / CON He 
Asparagin, CoHs(NH2)¢ Lee eens 4 gms. 
COOH 
Distilled water... .....0. 0.0... eee 1 liter 
Sodium hydroxide, NaOH.................. 20 c.¢. 
Sullivan’s (1905) Medium. 
; CON He 
Asparagin, CoH (NH) Leet e eee 10 gms. 
COOH 
Magnesium sulphate, MgSO4............... - 2 gms. 
Dibasic potassium phosphate, KoHPOs....... 1 gm. 
This medium supports good pigment formation by Ps. pyocyaneus 
and Ps. fluoresceous liquefaciens. 
Van Delden’s (1904) Solution. For SO4 reducing bacteria. 
Dibasic potassium phosphate, KeHPO....... 0.5 gm. 
Sodium lactate, CHs-CHOH—COONa...... 5.0 gms. 
Magnesium sulphate MgSOu................ 1.0 gm. 
/CONHs2 
Asparagin, Cels(NH2)C Lee eee ee 1.0 gm. 
COOH 
Ferrous sulphate, FeSO4..............000.. Trace 
Tap water... . 0.0... ccc cece cee cee cee aees 1000 e.e. 
This medium is of special value for the determination of sulphate 
reduction. 
