SYNTHETIC MEDIA 65 
Dolt’s Medium (1908). The following media were found to grow 
B. coli well in twenty-four hours. 
Medium [. 
CONH 
Asparagin, CoH (NH2)¢ ol eveeceees 1 gm. 
COOH 
Dibasic sodium phosphate, NazgHPQO,........ 0.2 gm. 
Distilled water............0.. 0000. c eee eee 100 c.c. 
Medium II. 
ASPATAQIN. 2... eee eee eee 1 gm. 
Dibasic ammonium phosphate (NH4)2eHPO,.. 0.2 gm. 
Distilled water... 0.0.0. .....0 00000 c cee eae 100 ¢.c. 
The following solid media were prepared: 
Purified agar, 3 per cent solution.... ....... 250 c.c¢. 
Asparagin........0... 0... c eee 2.5 gms. | 
NazHPO4g.......0 22. ee eee. 0.5 gm. 250 ©.c. 
Distilled water... ............., 100 c.c. 
Nitrogen-free Medium for Bacteria (Smith, 1905). 
Triple-distilled water...................05. 1000 c.c. 
Cane Sugar... eee eee 5 6.0. 
Monopotassium phosphate, KH2PO.......... 2 ¢.¢. 
Magnesium sulphate, MgSO4-7H2O......... 0.1 ¢.c. 
Sodium chloride, NaCl....................-. 0.5 ¢.c. 
All apparatus should be scrupuously clean and oily chemicals of 
known purity should be used. 
Dox’s (1910) Solution for Fungi. Modified Czapek’s Medium. 
(See Thom, 1910). 
Distilled water... 0.0.0... 00.0 cece eee ee eens 3000 c.c. 
Magnesium sulphate, MgSO................ 1.5 gms. 
Dibasic potassium phosphate, KeHPQ....... 3.0 gms. 
Potassium chloride, KCl................... 1.5 gms. 
Ferrous sulphate, FeSO4............-.....- .03 gm. 
Solidified media may be obtained by the addition of agar. 
