66 MEDIA AND THEIR PREPARATION 
Czapek’s Solution. Cultivation of the fungi. (From Smith, 1905.) 
Distilled water.........0. 0.00000. cee eee, 1000 e.c. 
Magnesium sulphate, MgSO4-7H2O......... .50 gm. 
Dipotassium phosphate, KoaHPO............ 1.00 gm. 
Potassium chloride, KCl..............000.. 0.50 gm. 
Ferrous sulphate, FeSO4g............0.0.00.. 0.01 gm 
Sodium nitrate, NaNOs... ¢............... 2.00 gms. 
Cane Sugar... cece cee eee 30.00 gms. 
Pasteur’s Medium (after Besson). 
Water... cece ce eee eens 100 gms 
Candied sugar..... 0... ....00. 00. cece eee 10 gms. 
Ammonium tartrate............... 0. 200, 0.1 gm. 
Ash of yeast... 0... 00.0.0 0.0 c eee .. ...0.075 gm. 
Raulin’s Solution for Molds. 
Distilled water......... 0.00.00. cece cece, 1500 c.e. 
Cane sugar, CigHe2011...2 220. 70 gms. 
Tartarie acid, (CHOH)2(COOH)2........... 4 gms. 
Ammonium nitrate, NH4NO3............... 4 gms, 
Ammonium phosphate, (NH4)2HPOs........ 0.6 gms. 
Magnesium carbonate, MgCQ3.............. 0.4 gm. 
Ammonium sulphate, (NH4)2SO4............ 0.25 gm. 
Zine sulphate, ZnSO4-7H2O0................ 0.07 gm. 
Tron sulphate, FeSOz-7H20... 2.000000... 0.07 gm. 
Potassium silicate, KSiO.........000.0000.. 0.07 gm. 
Lipman-Brown Medium (1910). 
Water... cece eee eee 1000 ¢.c. 
Dextrose, CeHi1206.........0.0....0000 cece 10.00 gms. 
Dipotassium hydrogen phosphate, KeHPOz... 0.50 gm. 
Magnesium sulphate, MgSO4-7H20......... 0.20 gms. 
Peptone.. 0.0.0.0... cece eee eee 0.05 gm 
FNS cs 20.00 gms 
Jordan’s Non-protein Medium. 
Redistilled water........ Dee cee ee 1000 c.c. 
Asparagin, CoH (NE) bt eens 2 gms 
COOH 
Magnesium sulphate, MgSO4:7H20......... 1 gm. 
Dipotassium hydrogen phosphate, K2AHPO.... 1 gm. 
