68 MEDIA AND THEIR PREPARATION 
MacKensie’s Culture Fluid (from Smith, 1905). 
Acid ammonium tartrate................4.. 1.5 gms. 
Bipotassium phosphate, KH2PO............. 2.5 gins. 
Potassium sulphate, KeS8O4..............0.. 1.5 gms. 
Sodium chloride, NaCl..................... 0.5 gm. 
Glucose, CeHi206... 0... ee 5.0 gms. 
Lactose, CrzHe2011. «2... ee ee 5.0 gms. 
Glycerol, CH2z2EOHCHOHCH2OH............ 15.0 gms. 
Distilled water........... 0.2.0... cee eee eee 1000 ¢.c. 
Alkaline to phenolphthalein. 
Proskauer and Beck’s Culture Fluid (from Smith, 1905). 
Commercial ammonium carbonate (NH4)2CO3 3.5 gms. 
Potassium phosphate, K3PO4............... 1.5 gms. 
Magnesium sulphate, MgSO4:7H2O......... 2.5 gms. 
Glycerol, CH. OHCHOHCH2OH............ 15.0 gms. 
Distilled water... 0.0.0... ... cece eee ee eee 1000 c.e. 
Fermi’s Culture Fluid. 
Distilled water.........00.0....0005. Lees 1000 c.c. 
Magnesium sulphate, MgSO4-7H2O......... 0.2 gm. 
Dipotassium hydrogen phosphate, HK2PO.4... 1.0 gm. 
Ammonium phosphate, (NH4)2sHPO4......:. 10 gms. 
Glycerol, CH2OHCHOHCH2OH............ 45 gms. 
This may be added to agar in place of peptone broth or to silicate 
jelly in which case the volume of H2O must be reduced. (Smith.) 
Fraenkel and Voges’ Solution (from Smith, 1905). 
Water............ occ e cece eee eens 1000 e.c. 
Sodium chloride, NaCl............... “eae 5 gms. 
Dipotassium phosphate, KoHPO,............ 2 gms. 
Ammonium lactate........... 0.0. ..0000 00. 6 gms. 
Sodium asparaginate............ 0.00.0. ce, 4 gms. 
Uschinsky’s Protein Free Medium. 
ASPAFAGIN. 6. cece een, 3.4 gms 
Ammonium lactate.........0.......0.005. 10.0 gms 
Sodium chloride, NaCl..............0.0..0... 5.0 gms. 
Magnesium sulphate, MgSO4-7H20...... 0.2 gm. 
Calcium chloride, CaCl...........0.0..... 0.1 gm. 
Dipotassium phosphate, K2aHPO,4.......... 1.0 gm. 
Distilled water... .....4....-.cccccececeee 1000 c.e. 
