80 STAINING TECHNIQUE 
Preparation of the Smear. The smeai on the slide o1 cover glass 
should be even and not too thick. For making this, a clean cover 
glass is removed from the bottle and allowed to diy in the air or by 
flaming By means of a sterile platinum loop place a small diop of 
sterile water on the slide and rub a little of the culture in it. It is 
best not to stir too much but by gentle tappings add enough of the 
growth to the drop of water. Spread the drop of water over the glass 
=a 
et ts 1 AV by 
ist 
Fia 32 —Types of Staining Jars and Dishes 
A, Coplin’s; B, Moore’s, C, Lellendahl’s, D, Naples Jar, E, Series of Jars for Differential 
Staining 
and allow to dry in the air or by gentle heating. After it has evap- 
orated fix by holding above the flame at a point which is not uncom- 
fortable for the hand. The procedure will give the smear. A good 
preparation should have the organisms evenly distributed over it 
and should not collect in droplets due to grease. The smear may 
now be subjected to any of the many staining processes. 
Staining Apparatus. While any method may be used to stain bac- 
teria certain pieces of apparatus are more convenient than others. 
When the organisms are mounted on a slide they may be covered 
