90 STAINING TECHNIQUE 
two forms. Each form has a dark or lilac-colored nucleus. The 
cytoplasm of one form is pale ‘blue and of the other form is blue with 
dark lilac or deep purple-colored granules, which are usually not so 
numerous as are the granules in the polynuclear neutrophilic leucocytes. 
The mast cells appear as cells of about the size of polynuclear leucocytes 
with purplish or dark-blue stained irregular-shaped nuclei and the 
cytoplasm sometimes bluish in which coarse spherical granules of 
variable size are imbedded. These granules are of a dark-blue or purple 
color and may appear almost black. The myelocytes have a dark 
blue or dark lilac-colored nuclei and blue cytoplasm in which numerous 
dark lilac or reddish lilac-colorea granules are imbedded. The blood 
olates are deeply stained and are & prominent feature of nearly all 
blood preparations.. They appear as blue or purple rounded or oval 
bodies, usually of a diameter of one-third to one-half of that of a red 
blood corpuscle. They have ragged margins and present fine bluish 
or purplish dots or mottlings in their substance. In some instances 
the blood plate appears to be within the red blood corpuscle or partially 
within and partially without. The plates found within the red cells 
must not be mistaken for young malarial parasites. 
Preparation of Common Stains. Alkalin Methylene Blue (Loeffler’s): 
Saturated alcholic methylene blue........... 30 .c¢. 
Aqueous KOH solution (0.1 per cent)........ 100 c.c. 
Carbol Fuchsin (Ziehl): 
Saturated alcoholic fuchsin solution......... 10 c.c. 
Carbolic acid erystals... 2... 0.0... ce eee. 5 gms. 
Distilled water... 0... eee eee ee ee eee 100 c.c. 
Antlin Gentian Violet: 
Saturated alcoholic gentian violet.......... 11 ¢.e. 
Absolute alcohol... 0.0.0.0... cee eee eee 10 c.c. 
Anilin water... 2... 0.0. cece eee 100 c.c. 
The anilin water may be made by shaking 5-6 c.c. of anilin oil in 
distilled water and filtering. 
Lugol’s Iodine Solution: 
