DIFTBERENTIAL STAINING OF BLOOD FILMS QO] 
Stabilized Gentian Violet (Stovall and Nicholls, 1916). To pre- 
vent the usual deterioration of gentian violet, the following stain is 
proposed: 
Aniline... 0... ccc eee ce eee eee 28 ¢.c 
Gentian violet... 0.0.0.0... cece cee eee eee 8 gms 
95 per cent alcohol ............000.00.000.. 100 c.e. 
Normal hydrochloric acid.... .... 0 ...... , 5 ¢.¢. 
Distilled water.................. 
The gentian violet should be dissolved in the alcohol. The aniline and 
hydrochloric acid may be mixed and diluted to 900 cc. Filter these 
solutions and add each other with a subsequent filtration. 
Another formula for gentian violet for use in the Gram stain has 
been given by Kilduffe (1909). This has been used in the author’s 
laboratory and has given good results. 
Solvent 
5 c.c. commercial formalin. 
95 c.c. distilled water. 
Stain 
25 c.c. saturated alcoholic gentian violet. 
75 c.c, of the solvent. 
or 
16 c.c. saturated gentian violet. 
84 c.c. solvent. 
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Barber, M. A. 1914. The Pipette Method in the Isolation of Single Micro- 
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BaumGarTen. 1911. Lehrbuch der pathogenen Mikroorganismen, p. 641, 
Original not seen. 
Bxcuroup. 1886. Fortschritt der Medizin, 1886, 4, 193. Original not seen. 
Brenstocx, B. 1886. Zur Frage der sogenannten Syphilis Bacillen und der 
Tuberkelfarbung. Fortschr. der Med., 1886. 193~195. 
Brunpny. 1908. Ueber die Beziehung der Farbbarkeit der Bakterien nach 
der Gram und ihrer Permeahbilitét. Cent. Bakt. Abt., IT., 21, 62. 
Butuiocu, W. and M’Lzop, J. J. R. 1904. The Chemical Constitution of 
the Tubercle Bacillus. Jour. Hyg., 4, 1-10. 
Drever, C., Krizcuer, 8. G., and Watxer, E. W. 1911. The Effect of 
Certain Dyes on Bacteria. Jour. Path. and Bact., 15, 133-1386. 
