200 PROTEINS AND CARBOHYDRATES 
condenser and heat slowly on a water bath until the fatty acids form a 
transparent layer on the surface. Cool to room temperature and add a 
few pieces of pumice stone and distill at a rate that will allow about 
110 c.c. to be driven over in thirty minutes. The pumice should be 
prepared by heating small pieces to a white heat and dropping them into 
distilled water. They should be kept under distilled water to keep 
them free from air. Mix the distillate, filter and titrate 100 c.c. with 
standard N/10 sodium hydroxide solution using phenolphthalein. 
To obtain the Reichert-Meissl number, increase the cubic centimeters of 
N/10 sodium hydroxide used by 100 c.c. of filtrate, by one-tenth, divide 
by the weight of fat taken, and multiply by 5. 
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