212 YEASTS AND MOLDS 
3. Blackish brown, viz.: 
(a) With branched sterigmata. A. miger (Cramer), van Tieghm, with 
sterile sclerotia; A. phenicis, Fat, and Delacr.; <A. strychni, 
Lindau; A. pulverulenta, MacAlpine; A. atropurpureus, Zimmer- 
mann; <A. volaceo-fuscus, Gasperini. 
(b) With simple sterigmata. A. luchuensis, Inui; A. calyptratus Oude- 
mans. 
4, Brownish yellow, yellow brown and reddish, viz.: 
(a) With simple sterigmata’ A. osteanus, Wehmer, A. WENTII WEHMER; 
A. perniciosus, Inui; A. giganteo-sulfureus, Saito; A. citrisporus, 
von Hohnel. 
(6) With branched sterigmata (occasionally associated with simple ones). 
A. sulfureus, Fresenius; A. ochraceus, Wilhelm (with sterile scle- 
rotia); A. rehmiz, Zukal (with ensheathed perithecia); A. spurius, 
Schroter; A. elegans, Gasperini; A. aurcomus, Gueguen (with 
sterile sclerotia). 
Examination of Molds. If molds are present in the foods or material 
under examination, they will appear on the agar plates after incubation 
although it may take a longer incubation period than for the bacteria. 
In the examination of foods or other substances where the entire flora 
is desired for study, dextrose agar should be used since this will permit 
the development of bacteria, yeasts and molds. These colonies should 
then be transferred to dextrose agar slants and, in this way, preserved 
for further study and identification. 
Total Organism. The structure and makeup of the total organism 
may be secured from a water mount on a plain slide or a hanging drop 
preparation. The water mount is made by carefully dispersing some 
of the mold mycelium in a drop of water on a slide. A cover glass is 
then dropped on the preparation after which it is ready for examination. 
Such a water mount will yield satisfactory results with objectives with 
the greater working distances. With the oil-immersion objective, it 
may be unsatisfactory on account of the very short working distance. 
From this preparation, the shape and makeup of the entire mold should 
be determined. 
Structure of Hyphe and Fruiting Body. The examination of this 
part of the fungus will require higher magnifications. Hanging-drop 
preparations of the hyphe should be prepared for examination with the 
oil-immersion objective. From this, the presence or absence of septa 
and other facts about the internal structure should be determined. 
The fruiting body should be carefully gone over in the same manner. 
The structure of the fruiting head, production of the spores, and other 
