BACILLUS TYPHOSUS IN FECES 255 
3 to 4 mm. in diameter. If typhoid-like colonies appear on the plates 
fish five or six of them, and inoculate tubes of Russell’s medium in the 
following manner: 
“Touch the colony with a sterilized platinum needle, make two 
streaks along the slanted surface of the medium in the tube and then 
stab the needle down through the center of the block of medium to the 
bottom of the tube. Incubate the inoculated tubes of Russell’s medium 
for twenty to twenty-four hours and then examine. 
Kendall and Day’s Method. “ The feces are collected preferably 
in a small rectal tube. A small portion of the feces (about a loopful) 
is thoroughly emulsified in 10 ¢.c. of sugar-free broth and preferably 
incubated one hour at 37° C. prior to the inoculation of the plates. This 
preliminary incubation does two things: the clumps of bacteria are 
thrown down, leaving a more uniform suspension of bacteria in the 
supernatant solution for inoculation and the bacteria undergo a slight 
development in a medium particularly suited for their growth. It is 
believed that better growth is secured if the feces is put through broth 
than if it is added directly to the solid media. It is essential that sugar- 
free broth be used. 
“The fecal suspension is then rubbed over the surface of the Endo 
agar plates in the usual manner by means of the stenle glass rods. 
These plates are then incubated at 37° C. for eighteen hours. At the 
end of this time small dewdrops are seen which may be removed entire 
to broth tubes. (1 ¢.c. broth which have been held at 37° C.) These 
are then incubated for two hours at the end of which time sufficient 
growth will have taken place, with which to make the agglutination 
tests.” 
Holt-Harris-Teague (1916) Method for Isolating B. Typhi from 
Stools. These authors have devised a medium which they claim will 
give better results than Endo’s medium. They claim that a greater 
percentage of colorless colonies on this medium turn out to be B. typhi 
than on Endo’s medium. It is prepared as follows: 
Nutrient agar is made in the usual way, containing 1.5 per cent agar, 
1 per cent Witte’s peptone, 0.5 per cent sodium chloride, and 0.5 per 
cent Liebig’s meat extract, to the liter of distilled water. It is cleared 
with egg-white, placed in flasks, and sterilized in the Arnold sterilizer on 
three successive days. The reaction is brought to +0.8. The agar is 
melted and saccharose (.5 per cent) and lactose (.5 per cent) are added. 
The medium is then heated for ten minutes in the Arnold. To every 
50 c.c. of the medium are added 1 ¢.c. of 2 per cent yellowish eosin and 
1 e.c. of 0.5 per cent methylene blue. We always add the eosin first 
