MICROSPIRA CHOLERA IN FECES 257 
He claims that positive results are more likely after preserving the 
stool in glycerol especially in hot climates or countries. 
Isolation of Typhoid Bacilli from Urine. (Morishama and Teague’s 
Method.) Streak two or three loops of the urine over an Endo or eosin- 
brillant green medium plate; add to the urine about one-half its volume 
of nutrient broth and incubate the mixture over night. If the plate 
which has been inoculated directly with the urine is negative prepare 
dilution of the incubated urine next morning and streak on the above 
plates again. ‘This latter procedure will furnish a higher percentage of 
positive results, 
EXAMINATION OF Feces FoR MicrosprrA CHOLERA 
Gedding’s Method. It would be neither possible nor desirable to 
fix a technique limited by strict rules for the various operations of bac- 
teriological examinations, but the following general indications may be 
recommended as permitting in the great majority of cases a positive 
diagnosis within twenty-four to thirty-five hours: 
1. When mucous flakes are available for examination of microscopical 
investigation of the same, in stained preparations and in the hanging 
drop. 
2. The isolation of the vibrios, employing for the purpose, agar 
media, at a temperature of 37° C. 
(a) Plant plates of ordinary suitably alkalinized agar and of Dieu- 
donne’s medium, using, for the latter, a risiform particle, or an equiva- 
lent quantity of feces. 
(b) Plant in 50 c.c. of peptone solution 1 c.c. of fecal matter. After 
a stay of six hours in the incubator (or twelve to eighteen hours, if need 
be), at 37° C. take several loopfuls from the surface and plant with them 
several plates of Dieudonne medium and ordinary agar. 
(c) Investigate the agglutination reaction, using drops for the pur- 
pose, from the isolated colonies, the properties belonging to cholera 
vibrios, and secure pure cultures. 
3. Demonstrate the character of the vibrios obtained in pure culture, 
by the reaction of agglutination or that of Pfeiffer. 
The conditions are much more favorable to the discovery of vibrios 
if pathological materials (feces or intestinal contents) are collected as 
early in the attack as possible, or secured from the cadaver as early as 
possible after death. Examinations made of the small quantity of 
material collected by a sound introduced into the rectum, in the living 
body, or from the cadaver are unreliable. It is sometimes possible to 
