258 INTESTINAL BACTERIA 
recognize that a person even in good health has undergone an attack of 
cholera by determining whether his blood serum gives with a genuine 
cholera vibrio the immunity reactions, viz., agglutination or the reaction 
of Pfeiffer. 
Teague and Travis’ Method. Two pounds of beef are soaked in 
2 liters of water over night. In the morning filter through cloth, heat 
in the Arnold steam sterilizer and filter through paper. Adjust the 
reaction to neutrality to litmus by means of sodium hydroxide. Inoc- 
ulate with B. cols and incubate for a few days. Then prepare nutrient 
agar from it by adding 1 per cent of Witte’s peptone, 0.5 per cent of 
sodium chloride and clear with egg white. Adjust the reaction to 0.5 
per cent alkaline, filter and add 0.25 per cent of nutrose. To 50 per cent 
of this nutrose agar add 1 per cent of sucrose and 1 c.c. of a 3 per cent 
solution of bluish eosin and 2 c.c. of a 1 per cent solution of Bismarck 
Brown. Mucrospira cholere colonies will show brown centers after 
twenty-four hours. 8B, cole will show pale pink or yellowish colonies. 
EXAMINATION OF Frces FoR B. TUBERCULOSIS 
Petref’s Method. In order to remove the food particles, dilute with 
water and filter through gauze. Saturate the filtrate with sodium 
chloride and, at the end of a half hour, all of the bacteria will be found 
in this film. Collect the film and add sodium hydroxide, shake well and 
incubate at 22° C. for three hours. Then neutralize to sterile litmus 
paper, centrifuge and inoculate. 
Reh Method. Stir the lump of stool in an Esbach glass with suffi- 
cient sterile water to make a soft paste but solid enough not to flow when 
the glass is tilted. Add a little ether and shake after closing with a 
rubber stopper. Pour the ether into a centrifuge glass and centrifugalize. 
The ether is then decanted and the sediment examined after staining 
with Ziehl’s method. 
Engleson’s Method. Scrape the rectal mucosa with an ordinary 
sound. Make a smear and stain. 
IsoLATION OF YEASTS FROM FECES 
Anderson’s Method. Prepare plates of Sabouraud’s agar. By 
means of a platinum wire which has been dipped into the feces emulsion, 
touch one of these plates in lines across the plate at distances of about 
4mm. Make as many rows as possible. The yeast colonies which 
develop in this way should be subjected to further study. 
