298 WATER HYGIENE 
produced in milk are identical. 
rently is in a chaotic state. 
TABLE XXVIII 
The nomenclature of this group appa- 
SHOWING THE ESSENTIAL CHARACTERISTICS OF SOME MEMBERS 
OF THE ANAEROBIC GAS FORMING GROUP OF BACTERIA 
(After Thresh, 1913) 
TI Bacillus 
Butyricus 
I Bacillus 
Entertidis sporagenes 
1. Cylindrical rods, on the)/1. Same as I. 
average 2.5-3.54 long, 
0.8--1.25u broad, stains 
well by Gram’s method 
some individual motile. 
2. Spores oval; stain after) 2. 
the several methods; 
situated in the middle 
of the rods more or less, 
1.64 long, In broad 
3. On gelatin grows well,| 3. 
softens rapidly and 
slowly liquefies. 
Grows well on the sur-/3. 
face of ordinary gela- 
tin as a translucent 
mass of convoluted 
threads; does not liq- 
uefy the gelatin. 
4. In stab gelatin, much}4. Forms spherical colon-|4. 
gas produced; spheri- ies with numerous hori- 
cal colonies without zontal filament  pro~ 
filamentous projec- jections; not lique- 
tions; slowly lique- fying; forms much gas, 
fying. 
5. On the surface of agar, |5. 
Gray round flat colo~|5. 
gray round flat colon- 
mes, margin thin and 
ies, few crenations, no much crenate, no 
spores. spores. 
6. Stab in agar, little tend-|6 Forms characteristic |6. 
ency for forming lat- 
eral branching, much 
gas, no spores. 
bundles of threads pro-~ 
jecting laterally from 
the growth in the 
stab, much gas, no 
spores. 
7. In_ milk, rapid separa-|7. Same as I. 7. 
tion of acid whey and 
flocculi of casein, small 
of butyric acid, no 
spores, much gas. 
8. Grows well on serum,|8. 
slowly liquefying, some 
spores found. 
9. Virulent for rodents. 
Grows well on serum, |8. 
very slow softening. 
9. Not pathogenic for ro-~/9. 
dents. 
1. Cylindrical and thread- 
Same as I, 2. 
III Bacillus 
Cadaverls sporogenes 
like, thinner and lon- 
ger than I and II, very 
motile, stains by 
Gram’s method. 
Spores oval, terminal 
drumsticks, stain after 
usual methods 1.6u 
long, lu broad. 
Rapidly liquefying, pu- 
trid odor, numerous 
spores formed. 
Much gas, rapidly lique- 
. fying, and putrid odor. 
Thready branched col- 
onies, with or without 
finely granular plate, 
rapidly forming spores. 
Much gas, rapidly form- 
ing spores, conspicu- 
ous masses of threads 
growing out of the 
stab. 
Milk is slowly decom- 
posed, putrid odor 
much gas, rapidly 
forming spores. 
Rapidly liquefying, pu- 
trid odor, rapidly form~ 
ing spores. 
Not pathogenic for ro- 
dents. 
Isolation of Bacillus Enteritidis Sporogenes. 
In examining water 
for this organism, it may be necessary to concentrate it either by fil- 
