TUBERCLE BACILLI IN MILK 413 
Campbell’s Method for the Detection of Tubercle Bacilli in Milk. 
Ten c.c. of a thoroughly mixed sample were placed in each of three 
centrifuge tubes by means of a sterile pipette. These should be cen- 
trifuged for thirty minutes at about 1200 r.p.m. The tubes are then 
removed from the centrifuge and the cream removed by means of 
a large sterile platinum loop. Three c.c. of sterile water should be 
added to this cream to bring the volume up for inoculation. The 
milk remaining in the centrifuge should be drawn down tole.c. These 
1 c.c. portions should be transferred to a sterile test tube. Slides may 
be made from these samples. Three c.c. of the cream emulsion and 
3 ¢.c. of the milk emulsion should be injected subcutaneously into guinea 
pigs. The pigs should be observed closely for from six to ten weeks and, 
if not dead before, should be autopsied. 
Anderson’s Method. Mix 50 c.c. of the milk with 50 c.c. of sterile 
water and centrifuge for one hour at 2000 r.p.m. Inject 4 c.c. of the 
sediment into a guinea pig. If the guinea pig dies, a careful autopsy 
should be made. If death does not occur by the end of two months, 
test with tuberculin, chloroform and perform a careful autopsy. All 
suspicious lesions should be cultured and cultural and morphological 
studies made. 
Besson’s Method for Isolation of Bacterium tuberculosis from Milk. 
Allow the fresh milk to stand for twenty-four hours and examine the 
deposit. Centrifuge and use the precipitate for making a microscopical 
examination. Coagulate 200 c.c. of the milk with a little citric acid and 
filter. Dissolve the precipitate on the filter in a solution of sodium 
phosphate (NaegHPOs+12H20) and pour the liquid into a large test 
tube. Add a few cubic centimeters of ether and, after shaking for 
about ten minutes, pour off the ether which will carry the fat with it. 
Centrifuge the aqueous fluid and examine microscopically the sediment. 
Beattie and Lewis (1913) have isolated acid fast bacilli from milk 
which were totally different from tubercle bacilli. They emphasize 
the futility of using microscopic methods instead of animal inoculation. 
The prevalence of tubercle bacilli in market milk has received the 
attention of investigators in practically all countries. There is an 
element of error in comparing closely data from the different investi- 
gations since a different number of samples were used. This may ex- 
plain the high incidence of tubercle bacilli which has been reported by 
some investigators. Table XLIII gives the data which have been se- 
cured by some of the investigators on market milk. 
Microspira Cholerze. Basenai (1895) reported that there was no 
germicidal action of milk on Microspira cholere and that the organisms 
