558 APPENDIX 
Dissolve the carbonate, citrate and thiocyanate in 800 ¢.c. of water 
and filter if necessary. Dissolve exactly 18 gms. of copper sulphate in 
100 ¢.c. of distilled water. Add this to the first solution with constant 
stirring after which add the 5 c.c. of potassium ferrocyanide and dilute 
to a liter. 
Diluting Fluid for Leucocytes. Prepare a 0.5 per cent solution of 
acetic acid and tinge slightly with gentian violet. 
Hayem’s Diluting Fluid for Erythrocytes. 
Sodium sulphate (NaeSO4)... 6.6.0.6... 22. 5 gms. 
Mercuric chloride (HgCle)................ 0.5 gm. 
Sodium chloride (NaCl)...............65. 1.0 gms. 
Distilled water... .. 02.0.0... 2c cee eee eee 200 c.c. 
Litmus or Azolitmin Solution. The standard solution of ltmus 
shall be a 2 per cent solution of reagent litmus in water. Crush the 
litmus in a mortar with a little water and, after diluting to the mark, 
boil for five minutes. Distribute in flasks or tubes and sterilize. This 
solution generally needs no correction but may be adjusted with dilute 
NaOH or HCl if necessary. 
Millon’s Reagent. Dissolve 30 c.c. of mercury in 570 c.c. of con- 
centrated nitric acid and dilute with two parts of water. 
Molisch Reagent. Dissolve 15 gms. of a-naphthol in 95 per cent 
alcohol. 
Nylander’s Reagent. Boil 4 gms. of potassium hydrogen tartrate 
and 2 gms. of bismuth subnitrate in 100 c.c. of 10 per cent KOH. When 
cool filter. 
Phenylhydrazine Mixture. Thoroughly mix two parts of sodium 
acetate and one part of phenylhydrazine hydrochloride. 
seliwanoff’s Reagent. Dissolve .05 gm. resorcinol in 100 c.c. df 
dilute HCl. 
Toisson’s Diluting Fluid for Erythrocytes. 
Sodium sulphate (NagSO4).......... eee eeee 8 gms. 
Sodium chloride (NaCl)............0.0000 08 1 gm. 
Glycerol .. 0... cee ccc eee neees 30 ¢.c. 
Distilled water... 0.0.0... 0... ccc eee ee eee ees 160 c.c. 
Color with gentian violet. 
Uffelmann’s Reagent. Add ferric chloride to phenol until a bluish- 
violet color results. 
