S8 



SOIL BACTERIOLOGY 



In the case of the inoculated cultures with nitrates present 

 proceed as follows: {a) Evaporate lo c.c, to dryness, and 

 (b) dilute lo c.c. to loo ex., and evaporate lo c.c. of this 

 to dryness (see page 143). 



7. From the results of the quantitative analyses fill in 

 the following table: 



Table 24..— Denitrification by Pure Cultures of Bacteria 





Treatment. 



Nitrogen in loo c c. of solution. 



No. 



As nitrate. 



Total nitrogen. 





Begin 



End 



Loss. 



Begin, 



End. 



Loss 



I 



2 



ControL 

 do. 



Mgm 



Mgm. 



Mgm 



Mgm. 



Mgm 



Mgm. 



3 



Unknown denitrifier. 

 do. 















s 



6 



Bacillus pyocyaneus. 

 do. 















7 

 8 



Bacillus Hartlebii. 

 do. 















9 

 10 



Bacillus coli. 

 do. 















Exercise 23 



Denitrification with the Formation of Nitrous Oxid 



(Optional) 



I. Prepare in glass-stoppered bottles medium 34 plus 

 80 grams potassium nitrate in each liter. 



