SOIL EXTRACT (FLAGELLATES AND CILIATES) g^ 



should be between (+0.8 or +1.0). It requires about 

 10 ex. of N/i NaOH per liter. In place of clarifying with 

 the white of egg, the author recommends heating for half 

 an hour at 15 pounds' pressure without disturbing the sedi- 

 ment and decanting through a cotton filter. 



Conn, H. J., BuL 38, New York Agr. Exp. Sta., 1914. 



COUNTING SOIL PROTOZOA 



Medium 11 



Hay-soil Extract 



Soil extract 100 c.c. 



Hay extract (o.i per cent, of dry hay) 100 c.c. 



Calcium carbonate (CaCOs) 5 gm. 



Tap-water 800 c.c. 



Medium 12 



Hay Infusion 



Hay 10 gm. 



Tap-water 1000 c.c. 



Medium 13 



Hay Egg-albumen (Ciliates) 



Hay 100 gm. 



Egg-albumen 5^ W^- 



Tap-water 1000 c,a 



Medium 14 

 Soil Extract (Flagellates and Ciliates) 



Soil extract loo.o c.c. 



Dibasic potassiiun phosphate (K2HPO4) 0.5 gm. 



Tap-water Qoo.o c.c, 



7 



