qS soil bacteriology 



Medium 15 



Mannit Solution 



See Culture-medium No. 39, page 108. 



AMMONIPICATION 



Medium 16 



Peptone Solution 



Peptone 10 gm. 



Distilled water 1000 c.c. 



Heat in the autoclave for thirty minutes and filter. 



Medium 17 

 Gelatin Solution 



Gelatin 



DistOled water. 



Sgm. 

 1000 c.c. 



Bring the reaction to (+1,0). 



Medium 18 



Casein Solution 



Casein 



Normal sodium hydroxid (NaOH) 



Distilled water 



10 gm. 



7 c.c. 



1000 c.c. 



Prepare according to directions on page 94. 



Medium 19 



Urea Solution 



Urea (COCNH^W ^ogm. 



Bouillon ( — i,o) 



1000 c.c. 



