EIO SOIL BACTERIOLOGY 



Medium 43 



Soil Extract 



Soil extract loo.o c.c. 



Dibasic potassium phosphate (K2HPO4) 0.5 grti. 



Mannit (C6H8(OH)6) lo.o gm. 



Distilled water 900.0 c.c. 



See note, page 95. 



Medium 44 



Saccharose Solution 



Saccharose (C12H22OU) . . 10,0 gm. 



Monobasic potassium phosphate (KH2PO4) i.o gm. 



Magnesium sulphate (MgSO* + 7H2O) 0.2 gm. 



Distilled water looo.o c.c. 



Medium 45 

 Maltose Solution 



Maltose (Ci2H220n + H2O) lo.o gm. 



Monobasic potassium phosphate (KH2PO4) i.o gm. 



Magnesium sulphate (MgS04 + 7H2O) o.i gm. 



Sodium chlorid (NaCl) trace 



Ferrous sulphate (FeS04 + 7H2O) trace 



Calcium chlorid (CaClg) fused trace 



Distilled water looo.o c.c. 



In order to prepare a solid medium, add 15 grams of agar to 

 each liter of the above solutions. 



Medium 46 



Bean-extract Caffein Agar 



Agar 15 gm, 



Caffein (C8H10N4O2 ■+• H2O) 2 gm. 



Dextrose (CeHiaOe) 20 gm. 



Bean extract 1000 c.c. 



