SOLUTION rOR CELLULOSE FERMENTATION III 



Note.—lBeeJi extract: Add to loo grams of powdered bean seed in a 

 mortar loo c.c. of N/i KOH. Allow this to stand a few minutes, then add 

 water sufficient to make 5 liters. This should stand twenty-four hours. 

 Siphon off the clear liquid, neutralize with phosphoric acid (H3PO4 + aq.), 

 and make the volume up to 5 liters. 



Zipfel, H., Centbl. Bakt. (etc.), Abt. 2, Bd. 32, pp. 107-131, 1911. 



Medium 47 

 Peptone Saccharose Solution 



Monobasic potassium phosphate (KH2PO4) 2.0 gm. 



Magnesium sulphate (MgS04 + 7H2O) o.i gm. 



Peptone i.o gm. 



Saccharose (C12H22O11) 20,0 gm. 



DistiUed water looo.o c.c. 



Buchanan, R. E., Centbl. Bakt. (etc.), Abt. 2, Bd. 22, p. 392, igog. 



CELLULOSE DESTROYING ORGANISMS 



{Anaerobic) 



Medium 48 

 Solution for Cellulose Fermentation 



Dibasic potassium phosphate (K2HPO4) i.o gm. 



Ammonium sulphate ((NH4)2S04) 1.0 gm. 



Magnesium sulphate (MgSOi + 7H2O) 0.5 gm. 



, Calcium carbonate (CaCOs) 2.0 gm. 



Sodium chlorid (NaCl) trace 



Distilled water , looo.o c.c. 



Fill large test-tubes about half-full. Add strips of filter- 

 paper. 



Omelianski, W., Centbl. Bakt. (etc.), Abt. 2, Bd. 8, p. 226, 1902. 



