Il8 SOIL BACTERIOLOGY 



Medium 60 

 Solution for the Oxidation of Thiosulphates 



Sodium thiosulphate (Na2S203 4" 5H2O) 5.0 gm. 



Sodium hydrogen carbonate (NaHCOs) i.o gm. 



Dibasic potassium phosphate (K2HPO4) 0.2 gm. 



Ammonium chlorid (NH4CI) o.i gm. 



Magnesium chlorid (MgCl2) + 6H2O) o.i gm. 



Tap-water looo.o c.c. 



Nathansohn, Mitt. a. d. zoolog. Station Neapel, Bd. 15, p. 655, 1902. 

 Beijerinck, W. M., Centbl. Bakt. (etc.), Abt. 2, Bd. 11, pp. 594-597, 1904. 



IRON ORGANISMS 



Medium 61 

 Solution for Thread Bacteria 



Potassium acetate (KC2H3O2) 0.5 gm. 



Ferrous carbonate (FeCOg) 0.5 gm. 



Tap-water looo.o c.c. 



Medium 62 

 Solution for Thread Bacteria 



Ferrous ammonium citrate (about 16 per cent. Fe) 0.5 gm. 

 Tap-water looo.o c.c. 



Medium 63 



Solution for Isolating Chlamydothrix 



(a) Agar lo.o gm. 



Manganese peptone (4 per cent. MnaOs) 0.5 gm. 



Tap-water looo.o c.c. 



(&) Gelatin loo.o gm. 



Manganese peptone (4 per cent. Mn203) 0.25 gm. 



Peat extract looo.o c.c. 



Make the reaction slightly alkaline with normal potassium 

 hydroxid. 



