122 SOIL BACTERIOLOGY 



Medium 73 

 Clover Agar 



Clover (green) 500.0 gm. 



Agar 25.0 gm. 



Saccharose (C12H22O11) 2.0 gm. 



Potassium nitrate (KNO3) 0.5 gm. 



Tap-water looo.o c.c. 



Extract the clover tissue by heating for one hour in the 

 steamer; filter and add the other ingredients. Add i to 2 

 drops of N/io hydrochloric acid to each tube of the medium 

 just before pouring plates. 



ACTINOMYCETES 



Medium 74 

 Solution for Actinomycetes 



Dibasic potassium phosphate (K2HPO4) 0.5 gm. 



Potassium nitrate (KNO3) 3.0 gm. 



Calcium malate (CaC4H405)2 + 6H2O) lo.o gm. 



Tap-water looo.o c.c. 



Krainsky, A., Centbl Bakt. (etc.), Abt. 2, Bd. 41, pp. 649-688, 1914. 



Medium 75 



Agar for Actinomycetes 



^^^^ 15.0 gm. 



Glycerin ,0.0 gm. 



Sodium asparaginate (NaC4H6N04 + H2O) i.o gm. 



Glucose (CeHiaOe) i.o,gm. 



Ammonium hydrogen phosphate (NH4H2PO4) .... i-S gm. 



Magnesium sulphate (MgS04 + 7H2O) 0.2 gm. 



Calcium cMorid (CaCy fused 0,1 gm. 



Potassium chlorid (KCl) o.i gm. 



Ferric chlorid (FeCls + 6H2O) trace 



Distilled water looo.o c.c. 



Conn, H. J., Jour. Bact, vol i, p. 198, 1916. 



