126 SOIL BACTERIOLOGY 



(b) Calcium nitrate (Ca(N03)2) 42.7 gm. 



Distilled water loco.o c.c. 



(c) Magnesium sulphate (MgS04 + 7H2O) 90 2 gm 



DistiUed water looo.o c.c ." 



(d) Ferric phosphate (FeP04 + 4H2O) 2.2 gm. 



Distilled water looo.o c.c. 



To prepare the complete nutrient solution, take 20-c.c. 

 portions of (a), (b), and (c) and dilute to i liter; now add 

 0.5 c,c. of solution (d). 



Shive, J. W., Physiological Researches, Vol. I, No. 7, Johns Hopkins 

 University, pp. 327-397, 19 15. 



Medium 84 

 Solution Similar to Sea-water 



Sodium chlorid (NaCl) 26.0 gm. 



Magnesium chlorid (MgCl2 + 6H2O) 3.7 gm. 



Potassium chlorid (KCl) i.o gm. 



Magnesium sulphate (MgS04 + 7H2O) 1.7 gm. 



Calcium sulphate (CaSOi + 2H2O) i.o gm. 



Distilled water looo.o c.c. 



This solution is recommended for the cultivation of organ- 

 isms accustomed to sea-water. 



Medium 85 

 Agar for Preserving Plate Cultures 



Washed agar 20 gm. 



Glycerin (C3H6(OH)3) 500 c.c. 



Distilled water 500 c.c. 



Dissolve the agar in the water by heating in a steamer, add 

 the glycerin, and filter through glass wool 



