DIRECTIONS FOR THE USE OE STAINS 129 



7. The mount should be labeled as follows: Name of 

 organism, stain, and date: 



9 txj 



H 2 • 





^§".>^ 





W P th g 





kj a p P* 





tcicola 

 Ifa. 



-violet 

 /16. 







« 





(a) Spread an even, thin film. 



(b) Fix in the flame. 



(c) Apply aniline oil .gentian-violet for two to five min- 



utes. 



(d) Wash in water. 



(e) Apply Gram's iodin for one minute, or until the 



preparation has a coffee color. 



(f) Wash in water. 



(g) Decolorize with 95 per cent, alcohol until no more 



violet color streams out. 

 (h) Wash in water. 



2. Endospore Stain (Double) : 



(a) Spread thin fihn. 



(b) Fix by passing through flame three times. 



(c) Stain with hot carbol-fuchsin five minutes (do not 



boil). 



(d) Clean under side of slide with 2 J per cent, acetic acid. 



(e) Decolorize the smear with 2I per cent, acetic acid 



until the pink color is nearly removed from film. 

 9 



