DIRECTIONS POR THE USE OF STAINS 13! 



5. LojSer's Flagella Stain: 



(a) Preparation of the Mordant — Dissolve 2 grains of 

 desiccated tannic acid in 15 ex. of distilled water 

 (heat gently), and add 5 c.c. of a saturated solution 

 of ferrous sulphate, i c.c. of an alcoholic solution 

 of fuchsin (i gram of fuchsin, 25 c.c. warm ab- 

 solute alcohol), I c.c. of I per cent, sodium hydrate 

 solution. Filter. 



(a) Preparation of Stain, — One part of a saturated alco- 

 holic solution of fuchsin to 4I parts of a 5 per cent, 

 solution of carboHc acid. 



(6) Filter. 



(a) Method of Staining. — Mordant one minute. 

 (&) Wash in distilled water. (Have water in wide- 

 mouthed beaker.) 



(c) Stain with carbol-fuchsin one-haK minute (heat 



gently). 



(d) Wash in water. 



(e) Dry thoroughly. 

 (/*) Treat with xylol. 

 (g) Mount in balsam. 



6. Zettnow's Flagella Stain: 



(a) Solution (i): Dissolve 2 grams of tartar emetic 

 (2K(SbO)C4H406 + H2O) in 40 c.c. of water. 



(J) Solution (2): Dissolve 10 grams of tannic acid 

 (C14H10O9) in 200 c.c. of water. 



(c) Warm solution (2) to 50° to 60° C, add 30 c.c. of the 



tartar emetic solution (i). The turbidity of the 

 mordant should entirely clear up on heating, 



(d) Next dissolve i gram silver sulphate in 250 ex. 



distilled water. 



