Kiorate 



The lima bean straw was blended with chloroform, filtered, and the fil- 

 trate dried with sodium sulfate, A portion of the extract was diluted to 

 100 ml. with chloroform and washed twice with 10 ml.. of 75 percent sulfuric 

 acid followed by 3 water washes and a saturated sodium chloride wash. The 

 extract was cleaned up by chromatogiraphing through a dry column of 3:1 acid- 

 washed Nuchar-C-190-N:Hyf lo Super Cel. The phorate content was determined by 

 the method of Giang and Schechter (10) . 



Trichlorfon 



In the analysis of trichlorfon residues the straw samples were blended 

 with chloroform and filtered through anhydrous sodium sulfate. A portion of 

 the extract was evaporated to a small volume and transferred to a test tube 

 containing 1/2 ml. of water. The chloroform phase was evaporated and the 

 trichlorfon residues determined by the inhibition procedure of Hensel and 

 associates.— 



Range Grass 



Bomyl 



The sample of range grass was ground dry in a blender, transferred with 

 chloroform to a tumbling Jar, allowed to steep for 24 hours, tumbled, and the 

 extract removed by filtration. A portion of the extract was concentrated to 

 30 ml. and chromatographed through a dry column of a mixture of equal parts 

 Nuchar-C-190-N, aluminum oxide, magnesium oxide-Hyflo Super Cel (1:1) and 60- 

 mesh Florisil. The column was eluted with chloroform and the sample evapo- 

 rated to dryness. For original portions of 20 ml. or less, the samples were 

 shaken with 2 g. of the above mixture rather than chromatographed through the 

 column. The Bomyl residue was determined by the total phosphorus method of 

 Saliman (25) . 



Carbaiyl 



The range grass samples were extracted in the same manner as the red 

 clover samples. A portion of the extract was treated with the coagulating 

 mixture of Miskus, Gordon, and George ( 18) , evaporated to about 15 ml. , and 

 shaken with an Attaclay-aluminum oxide mixture (1:1), filtered, and the 

 carbaryl residues were determined by the method of the Union Carbide and 

 Chemical Corp. ( 28) . 



Diazlnon 



The samples were tumbled with petroleum ether and the extract was fil- 

 tered through sodium sulfate. No cleanup procedure was required. The method 

 for the determination of sulfur as published by Gigger (12) was used in the 

 final analysis. 



5/ See footnote No. 4. 



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