Table 54.— Protein, fat, and ash in kidneys from rats fed other experimental diets 



Strain and diet 



Rats 



Aver- 

 age 

 age 



Aver- 

 age 



weight 

 loss 



Aver- 

 age 

 kidney 

 weight 



Water 



Based on dry weight 



Pro- 

 tein 



Fat 



Ash 



Total content 



Pro- 

 tein 



Fat Ash 



BHE rats 

 SPE supplemented with — 



Choline, 0.5%- 



B^, 0.01 mg./lOO gm__ 



Choline, 0.5%+ B^, 0.01 rag./ 



100 gm 



B 6 , 0.5 mg./lOO gm 



Choline, 0.5 % + B 6 , 0.5mg./100sm 

 Choline, 0.5% + B 12 , 0.01 mg./lOO 



gm.+ B 6 , 0.5 mg./lOOgm 



Cholesterol, 0.46% 



Cholesterol, 1.38% 



Ascorbic acid, 0.2% 



Ascorbic acid, 0.2% + choles- 



terol, 0.46% 



SP 16 HVO 



SP 8 lard 



SP 16 lard 



SP 8 butter 



SP 16 butter 



E100 



Y100 



Littermates fed — 



SPE 



Y100 



Y97 + salt mixture. 3.0% 



Diet reversal: 



Sacrificed at approx. 250 davs: 



Stock 



SPE 



Continued on — 



Stock 



SPE 



Reversed at 250 davs: 



Stock changed to" SPE 



SPE changed to stock 



Wistar rats 



SP 8 HVO. 

 SPE 



A T wm- 

 ber 

 19 

 8 



10 



7 

 9 



in 

 8 



Id 

 9 



11 

 9 

 8 

 9 

 9 



14 



5 



4 

 4 



Days 

 492 

 463 



434 

 502 



412 



431 

 451 

 409 

 415 



434 

 629 

 557 

 572 

 601 

 486 

 559 

 375 



428 

 420 

 424 



\Grams 

 139 

 131 



114 

 129 

 127 



129 

 150 

 142 

 115 



1S3 

 84 

 99 



117 

 82 

 72 



141 

 80 



t',6 



42 



6 



250 



249 



590 

 396 



686 

 577 



762 

 772 







(i 



195 

 133 



109 



116 



119 

 103 



Grams 



6. 33 



7. 11 



7. 18 

 5. 13 



4. 74 



5. 76 

 7.54 



6. 83 

 5. 00 



74 

 76 

 26 

 31 



OS 



2. 74 



4. 3ti 



2. 42 



3. 88 

 2. 54 

 1. 66 



2. 07 



2. 74 



4.25 

 4.64 



3. 94 

 3. 55 



2. 02 

 2. 80 



Per- 

 cent 

 82. 1 

 81.8 



82.3 



79. 5 



80. 5 



81. 4 

 81. 5 



81. 6 

 80. 1 



80.2 



77. 9 

 79. 5 

 79. 7 

 79. 3 



78. 



82. 9 



79. 4 



79. 5 



80. 2 



78. 8 

 81. 3 



81. 3 



84. 5 



SO. 9 

 SO. 6 



80.0 

 79. 6 



Per- 

 cent 

 85. 4 

 78.8 



82. 5 

 82.0 



82. 8 



80. 8 



83. 2 

 85. 3 

 83. 



85. 1 

 78.9 

 82. 2 

 82. 2 

 SO. 5 

 79. 3 

 82. 7 

 79. 5 



83.5 



79. 4 



80. 8 



79. 8 

 76.8 



84. 7 

 78. 8 



84. 2 



82. 4 



82. 

 80. 9 



Per- 

 cent 

 9.0 

 9.6 



10.3 



9. 3 



10. 2 



10. 3 



11. 



10. 1 

 8. 6 



9. 9 

 13. 4 

 11. 6 

 13. 2 

 12 

 13 



4 

 5 

 13. 6 



13. 7 



9. 9 



14. 6 

 13. 5 



13. 2 

 12. 8 



8. 1 



9. 3 



9. 2 

 9. 5 



13. 5 

 16. 4 



Per- 

 cent 

 5. 9 

 5.2 



5. 8 

 5. 6 



5. 4 



6. 6 

 5. 1 

 6.8 

 5. 2 



6.0 



4. 7 

 4. 2 

 4. 7 

 4. 

 4. 8 

 5.6 

 6.2 



6.5 



6.0 

 5.7 



3. 

 2.0 



5.9 



4.9 

 3.8 



4. 3 

 4.3 



Ma. 

 950 

 954 



963 



824 

 724 



896 



1,240 



971 



802 



940 

 446 

 540 

 529 

 400 

 459 

 523 

 363 



637 

 383 

 299 



341 

 375 



654 

 565 



624 

 560 



323 

 446 



Mg. 

 95 

 105 



112 

 89 

 80 



110 



155 



109 



76 



109 

 71 

 71 

 81 

 59 

 69 

 86 

 61 



70 



69 

 50 



Mg. 



67 

 69 



71 



58 

 49 



72 

 76 

 78 

 50 



67 

 26 

 26 

 30 

 20 

 29 

 35 

 31 



54 

 29 

 21 



56 



61 



13 

 10 



63 



67 



41 



67 

 63 



35 

 26 



54 

 89 



18 

 23 



kidney and a high percentage of ash did not 

 necessarily paraUel one another. 



Differences between the composition of kidneys 

 from BHE and Wistar rats were due chiefly to 

 differences in the kidney weights of these two 

 strains of animals. 



Urinary protein 



BHE rats. — Data on urinary protein excretion 

 were obtained for BHE rats fed 11 of the diets 

 under investigation, and the results are sum- 

 marized in table 55. Urine was collected under 

 two conditions: (1) a 7-hour collection period 

 without access to food; (2) a 17-hour collection 

 period with access to food. The conditions of 

 collection are indicated in the table, and the urinary- 

 protein values recorded are for total protein 

 excreted during the collection period. Results 



are grouped by age at which urine collection was 

 made; in most cases the age range within groups 

 was less than 2 weeks. Data for age at death 

 and size of kidney are included to relate as well as 

 possible the protein excretion with age at death, 

 although urine samples were not collected at 

 this time. 



The amount of protein excreted by rats under 

 200 days of age was generally small regardless of 

 diet. When urine collections were made for a 

 7-hour period without access to food, protein 

 excretion was less than 10 mg. for 67 percent 

 of the rats. Only 3 of the 61 rats in this group 

 excreted more than 50 mg. of protein. 



When data obtained under comparable con- 

 ditions were available, a tendency for increased 

 protein excretion with increasing age was apparent. 

 Dietary differences were observed in the older 



66 



