Table 21. — Liver fat determined microscopically for rats fed other experimental diets 



Strain and diet 



Rats 



Average 

 age 



Average 



weight 



loss 



Histological rating of 

 liver fat 





Small 

 vacuoles 



Large 

 vacuoles 



BHE rats 



SPE supplemented with — 



Choline, 0.5% _ ... 



Number 



22 

 7 



10 

 8 

 8 



10 

 8 

 9 

 9 

 9 

 5 



15 

 8 

 6 

 9 



17 

 9 

 6 

 7 

 5 



20 



16 



5 

 5 

 5 



3 

 3 

 4 

 3 



9 

 9 



5 

 9 



7 



Days 

 466 

 449 

 434 

 472 

 408 

 430 

 451 

 406 

 415 

 434 

 547 

 553 

 547 

 546 

 519 

 698 

 632 

 530 

 474 

 550 

 535 

 403 



428 

 425 

 430 



590 



473 

 686 

 577 



729 

 664 



886 

 703 

 500 



Grams 



144 



136 



113 



126 



123 



139 



150 



142 



115 



183 



76 



99 



120 



76 



69 



173 



118 



155 



178 







135 



77 



66 



35 



5 



195 

 104 

 109 

 101 



106 



78 







75 

 85 



Score 



1. 1 

 . 4 

 . 4 



1. 1 

 . 8 



1. 2 



1.3 



1. 6 

 . 6 

 . 4 





 . 1 

 . 1 

 . 5 





 _ 2 



'. 2 

 .2 

 . 9 

 . 2 

 . 2 

 . 8 



1. 2 

 1. 

 1. 2 





 



1.0 

 



. 1 

 . 4 





 . 2 

 1. 4 



Score 

 0. 5 



Bjo, 0.01 mg./lOO gm _ . ._ ... _. 



. 6 



Choline, 0.5%+Bi., 0.01 mg./lOO gm 



. 6 



B 6 , 0.5 mg./lOO gm. . _ . 



. 9 



Choline, 0.5%+B 6 , 0.5 mg./lOO gm. . . ___ .. ._ 



. 6 



Choline, 0.5%+B 12 , 0.01 mg./lOO gm.+ B 6 , 0.5 mg./lOO gm 



Cholesterol, 0.46% .. ... ... . 



. 9 

 2. 7 



Cholesterol, 1.38%. . - - ... 



2. 1 



Ascorbic acid, 0.2% 



. 8 



Ascorbic acid, 0.2%+ cholesterol, 0.46% 



1. 



SP 16 HVO 







SP 8 lard 



.3 



SP 16 lard 







SP 8 butter 



. 5 



SP 16 butter. _ ...___ 



. 1 



SPa 16 HVO.. 



1. 5 



SPb 8 HVO 



. 6 



SPEW 



0. 5 



SPEY . .. 



1. 3 



SPW 8 HVO .. 



. 4 



E100 .. 



. 8 



Y100 



. 5 



Littermates fed — 



SPE 



. 4 



Y100 



. 8 



Y97+ salt mixture, 3.0-%--. __ .. . _.. _. . __ 



.8 



Littermates fed — 

 Stock _ 







SPE 



. 7 



Stock changed to SPE at 250 davs . . 



1. 



SPE changed to stock at 250 days. . 



. 3 



Wistar rats 

 Littermates fed — 



SP 8 HVO 







SPE 



. 7 



BHE parents fed Wistar stock diet 

 Young fed — 



Stock 







SP 8 HVO 



. 2 



SPE 



1. 4 







SP 8 HVO rats. More than 50 percent of the 

 kidneys from rats over 300 days of age exceeded 

 2.0 grams in weight. In nonfasted animals fed 

 this diet, kidneys tended to increase in size with 

 age. Two of the rats 300 to 399 days old had 

 kidneys weighing 8.9 grams. 



Liver weight of animals maintaining weight 

 on stock, SP 8 HVO, and SPE diets.— In table 

 23 are summarized comparable data for the livers 

 from rats fed stock, SP 8 HVO, or SPE diets. On 

 the stock diet, there was no indication that age 

 influenced appreciably the size of the livers from 

 fasted rats. The average liver weight for these 37 

 rats was 11.1 grams, representing 2.6 percent of 

 body weight. 



The livers from nonfasted rats tended to increase 

 in size with age, representing 3.4 percent of body 



33 



weight in rats less than 200 days old and 3.9 

 percent in rats 600 days of age and older. The 

 livers from these nonfasted rats were heavier than 

 those from the fasted rats, and the extent of the 

 differences in the weights of this organ between 

 these two groups of rats depended upon age. 

 Livers from nonfasted rats less than 300 days 

 old were approximately 30 percent heavier than 

 those from fasted rats of comparable age. In 

 rats over 500 days old, the difference amounted to 

 approximately 60 percent. The range of values 

 for liver weights of the nonfasted rats was wide, 

 even within age groups, with an extremely wide 

 range of values for rats exceeding 300 days of age. 

 The results for the distribution seen in table 23, 

 however, show that the increase in liver size of 

 nonfasted rats with age was due to the increase in 



