damage was generally present, regardless of age. 

 Kidneys from rats fed SPM diet snowed the least 

 evidence of degenerative changes. In spite of 

 extensive damage, calcium deposits were rarely 

 seen in the kidneys of rats fed SPPB diet although, 

 otherwise, these kidneys were similar to those 

 from comparable animals fed SPE diet. With 

 regard to calcium deposition, the kidneys from 

 rats fed SPB diet resembled more closely those 

 from rats fed SPE diet. 



Rats fed other experimental diets. — In 

 table 18 are summarized the results of micro- 

 scopic examination of the kidneys from rats fed 

 all of the other experimental diets. The data 

 were obtained chiefly from rats that were sick 



or moribund. The influence of weight loss on 

 the extent of kidney damage was seen consist- 

 ently in all of the experimental series, but for 

 most diets the data were too limited to permit a 

 separation of the results on this basis. The 

 results reported, therefore, include all available 

 data, along with the average weight loss for each 

 group to aid in comparing the results of the 

 various experimental regimens. 



None of the supplements investigated were able 

 to prevent extensive degenerative changes that 

 occur in the kidneys of rats fed SPE diet. How- 

 ever, the data summarized in table 18 do show 

 some trends suggesting that these supplements 

 may have influenced the metabolic processes 



Table 18. — Kidney damage determined microscopically for rats jed all other diets 



Strain and diet 



Rats 



Average 

 age 



Average 



weight 



loss 



Histological rating of kidney 





Hyalin 



Cystic 



Glomerular 



Calcium 



BHE rats 



SPE supplemented with — 



Choline, 0.5%. ._ ._-.__ 



Number 



22 



7 



10 



8 



8 



10 

 8 

 9 

 9 

 9 

 5 



15 

 8 

 6 

 9 



17 

 9 

 6 

 7 

 5 



20 



16 



5 

 5 

 5 



3 



2 

 4 

 3 



9 

 9 



5 



9 



7 



Days 

 466 

 449 

 434 

 472 

 408 



430 

 451 

 406 

 415 

 434 

 547 

 553 

 547 

 546 

 519 

 638 

 632 

 530 

 474 

 550 

 535 

 403 



428 

 425 

 430 



590 

 396 

 686 



577 



729 

 664 



886 

 703 

 500 



Grams 

 144 

 136 

 113 

 126 

 123 



139 



150 



142 



115 



183 



76 



99 



120 



76 



69 



173 



118 



155 



178 







135 



77 



66 



35 



5 



195 

 133 

 109 

 101 



106 



78 







75 

 85 



Score 

 2.2 

 2.0 

 2. 4 

 2.0 



1. 8 



2.2 



2. 2 

 1. 8 



1. 8 



2. 2 

 1.2 

 1.8 

 1. 8 



1. 8 

 1.7 

 1.6 

 1.7 



2. 5 

 2.3 



.6 



1.5 



.6 



2. 4 

 . 8 

 . 4 



2.7 

 2.0 

 2.2 

 2.0 



.4 

 .6 



. 8 

 1.7 

 2. 1 



Score 

 2. 8 

 3.0 

 2.9 

 2.0 

 1.9 



2.6 



2. 8 



2. 7 

 2.3 

 3.2 



.2 

 1.4 

 1. 1 

 .7 

 . 7 

 1.5 

 1.0 

 1.8 



3. 1 

 

 2.3 



.9 



1.4 

 .6 

 



2.7 

 3.5 

 1.8 

 1. 7 



.3 

 . 6 



. 2 

 1.0 

 2.3 



Score 

 1.5 



1. 9 



2. 

 1.2 

 1. 2 



1. 4 

 1.2 

 1. 3 

 1. 1 

 1.7 



.4 

 1. 2 

 1. 1 



. 8 



. 6 

 1.2 



.9 

 1. 

 1.6 

 

 1.4 



. 2 



1.0 



. 4 

 



3.0 

 1.5 

 1.0 



1. 7 



. 2 

 . 4 



.4 



. 8 



1. 4 



Score 

 1. 



B 12 , 0.01 mg./lOO gin _ _ .. ______.. 



1. 3 



Choline, 0.5% + B 12 , 0.01 mg./lOO gm. 



. 8 



B 6 , 0.5 mg./lOO gm 



.4 



Choline, 0.5% + B 6 , 0.5 mg./lOO gm 



. 6 



Choline, 0.5%+Bi 2 , 0.01 mg./lOO gm. + B„, 

 0.5 mg./lOO gm... ... 



. 3 



Cholesterol, 0.46%. . 



. 4 



Cholesterol, 1.38% . 



. 1 



Ascorbic acid, 0.2% . _ _ 



1. 7 



Ascorbic acid, 0.2% + cholesterol, 0.46% 



SP 16 HVO 



1. 6 

 



SP8 1ard 



. 5 



SP 16 lard 







SP 8 butter 



. 5 



SP 16 butter 



. 4 



SPa 16 HVO 



. 5 



SPb 8 HVO 



. 3 



SPEW 



1. 3 



SPEY 



SPW 8 HVO 



1.9 

 



E100 



. 6 



Y100 



1. 6 



Littermates fed — 



SPE 



Y100 



. 8 

 2.0 



Y97 + salt mixture, 3.0%. ... .... 







Littermates fed — 



Stock 



SPE 





 1. 5 



Stock changed to SPE at 250 days. . _ _ 



. 8 



SPE changed to stock at 250 days 



Wistar rats 



Littermates fed — 



SP8HVO 



.3 

 



SPE 







BHE parents fed Wistar stock diet 



Young fed- 

 Stock .... ... 



. 8 



SP8 HVO 



. 2 



SPE 



. 6 







33 



