Vaccinating 



Some States prohibit vaccination and consider vaccinated herds infected, placing 

 them under quarantine. Check with your State animal health official or APHIS-VS 

 Area Office before initiating any vaccination program. 



Instructions for Testing 



Step 1 : Preparation — Because pseudorabies can be spread oropharyngeally, it is 

 important to disinfect the snare between hogs, to leave the snare and other equipment 

 on the farm, and to disinfect your own equipment carefully. See the section entitled 

 "Selecting and Using Disinfectants." 



1. When a herd is tested for the Cooperative Pseudorabies Eradication Program, the 

 State animal health official of the APHIS-VS Area Office will provide you with the 

 appropriate sample size and selection needed for each herd. 



2. The key to testing is to provide a randomly selected sample. 



Step 2: Identifying Animals — 



1. All test animals must be individually identified by tattoo, plastic bangle tag, 

 or other official identification. Refer to the section entitled "Current Animal 

 Identification" for further information. 



Step 3: Collecting Blood Samples — 



1. Use only clean tubes for swine blood collection. 



2. Collect a minimum of 3 mL of whole blood. 



3. Because swine blood hemolyzes easily, separate the serum from the clot before 

 mailing. Most laboratories prefer at least 1 mL of serum for testing. 



4. Refi-igerate the sample if shipping will be delayed. Avoid shipping over a weekend. 



5. The samples must be tested by an approved laboratory. 



Step 4: Recording the Results of the Test — 



1. Send completed forms with the samples. If your State has no standard 

 pseudorabies test form, use VS Form 4-33, Brucellosis Test Record. 



2. If the herd you are testing is using vaccine, make sure you clearly indicate the 

 vaccine type and manufacturer on the test chart. 



3. At the top of VS Form 4-33, delete brucellosis and print PSEUDORABIES; also 

 print PSEUDORABIES in the Remarks block. Any questions should be directed to 

 your State animal health official or APHIS-VS Area Office. 



Complete the blocks of VS Form 4-33 as shown in appendix D. 



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