5. Use caution. RB51 may cause clinical brucellosis in humans if accidentally 

 injected, sprayed in the eyes, or allowed prolonged contact with the skin. If you 

 are exposed, contact a physician as soon as possible. 



6. After reconstitution, the vaccine loses potency rapidly. Do not reconstitute more 

 vaccine than will be used in 1 hour, and, if working in warm weather, keep the vial 

 on an ice pack to maintain viability. 



7. To avoid contamination and accidental vaccine exposure to other than 

 vaccination-eligible animals, maintain separate syringes and needles for 

 brucellosis. 



8. Administer 2 ml. of the vaccine subcutaneously. 



Step 2: Tattooing — 



1. Clean the inside of the right ear to enhance ink penetration. Green ink works best 

 for legibility, especialK' in black-pigmented ears. 



2. Tattoo the ear with the appropriate coding between cartilage ribs in the middle 

 of the ear. Allow tor normal growth ot the ear. It ear marks or notches do not 

 permit this location, tr\ to place the tattoo as near as possible to the recommended 

 position. 



3. Apply the ink with a dauber and rub the ink into the holes well with \'our thumb 

 or an old toothbrush. 



4. Vaccination tattoos must be applied to the right ear. For B. abortus Strain 19 

 vaccinates, the tattoo will include the U.S. Registered shield and "V," which will be 

 preceded by a number indicating the quarter of the year and followed b) a number 

 corresponding to the last digit of the year in which the vaccination was done. 



For B. abortus Strain RB51 vaccinates, the tattoo w ill include the U.S. Registered 

 shield and "V," which will be preceded by a letter R and followed by a number 

 corresponding to the last digit ot the year in which the vaccination was done. 

 Documentation ot brucellosis vaccination tattoo information on the VS Form 

 4-33 is essential for accurate test interpretation. 



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