All isolates produced yellow-orange pigment when exposed to white light but remained white when incubated 

 in the dark. 



Carotene crystals were demonstrated in each of the isolates on 7H10 medium 3 to 4 weeks after inoculation. 



Crowth from all isolates appeared in approximately 14 days when subcultured at 37 C, on Lowenstein-Jensen 

 medium. No growth was observed at room temperature or 45 C. 



The biochemical characteristics of the isolates were determined by tests generally accepted for differentiating 

 mycobacteria (3, 10, 2) and are tabulated in table 1. 



Table 1.— Biochemical reactions of three M. KANSASII strains isolated from cattle 



Biochemical test 



Case number 



716231 



723150 



727630 



P/B Medium 



Nitrate Reduction 



Catalase 20° 



Tween 80 Hydrolysis (24 hrs) 



Arylsulfatase (3 days) 



Tellurite Reduction 



5% Salt Tolerance 



INH (10 ug) 



TCH (15 ug) 



Neotet. 1 :40000 



Streptomycin (2.0 ug) 



Rifampin (.025 ug) 



Niacin 



— negative ++ moderate reaction 



+ weak reaction I I I strong reaction 



I I I I very strong reaction 



Uniform 



+-H-+ 

 I I I I 



Uniform 



Uniform 



++-H- 



+++ 



++ 



R 



R 



R 



R 



R 



R 



R 



R 



R 



R 



S 



R 



R 



R 



R 





R = Resistant 







S = Sensitive 





Discussion 



The isolates described represent typical M. kansasii strains in that characteristic carotene crystals were 

 observed and Tween 80 was hydrolyzed within 24 hours. These tests are definitive for this species. 



M. kansasii has been isolated from humans but reported isolations from animals have been infrequent. 



From the histopathologic data, it can be seen that no definite observations of acid-fast organisms were found 

 in tissue. All tissues showed some aberrant changes - the cause of which is not known. 



REFERENCES 



1. Chapman, J. S. et. al. Amer. Rev. Resp. Dis., Vol. 91, p. 351, 1965. Isolation of Mycobacteria from Raw Milk. 



2. Jarnagin, J. L., et. al. American Rev. Resp. Dis., Vol. 104, p. 763, 1971. The Isolation of M. xenopi from 

 Granulomatous Lesions in Swine. 



3. Karlson, A. C, et. al., Mayo Clinic Proc, Vol. 39, P. 410, 1964. Identification of M. tuberculosis with One 

 Tube of Liquid Medium. 



4. Lipe, R. S. et. al. 4th Semi Annual Progress Report of the Research Contract with the USDA and Michigan 

 State Univ., p. 121,1961. 



5. Mailman, W. L., Progress Report on the Research Contract Between the USDA and Michigan State Univ., 

 p. 20, 1960. 



6. Mailman, V. H. et. al. Health Laboratory Sci., Vol. 1, p. 11-20, 1964. Relationship of Atypical Bovine and 

 Porcine Mycobacteria to Those of Human Origin. 



35 



