PROCEDURE 



One cellar in each of two wineries was used for all tests. Both wineries were 

 located within 2 miles of the laboratory at Fresno, Calif. , in the northeastern section 

 of the city. Both cellars were similarly constructed with brick walls and wooden roofs. 

 Both had screened windows, but the screens in the large cellar did not keep the vinegar 

 flies outside. Doors in both cellars were occasionally left open for the passage of 

 hoses used in unloading the wine tanks. 



The smaller cellar, used in 1964 and 1965, contained 29 closed wooden wine 

 tanks with capacities varying from 1, 000 to 32, 000 gallons of wine. It contained 

 161,284 cu. ft. of air space. The larger cellar, used in 1964 and 1966, contained 

 32 closed wooden wine tanks, each tank capable of holding 50, 000 gallons of wine. 

 This cellar contained 268,495 cu. ft. of air space. 



The semiautomatic vapor generator that dispenses the dichlorvos was developed 

 by the Stored- Product Insects Research and Development Laboratory at Savannah, 

 Ga. 5/ This machine produced dichlorvos vapors by heating 30 pounds of resin pellets 

 impregnated with 20- percent dichlorvos. A fan increased the dispersal of the vapor. 

 The machine was operated at a temperature of 120 ± 5 * F. The airflow regulator was 

 set at a pressure of 0. 5 inch of water. 



The vapor generator was operated from 1 to 1 1/2 hours during each test in 

 1964. During the first and second tests, it was operated from several locations at 

 one end of the cellar. However, the experimenters decided that the machine would 

 perform efficiently from one location, and all subsequent tests were made in this 

 manner. 



Insect cages were located in the four corners of the cellar 6 feet or 24 feet 

 above the floor. In the first and second tests, an additional cage was placed 24 feet 

 above the floor on top of a wine tank in the center of the cellar. 



In the 1965 test, the machine was placed at one end of the smaller cellar. The 

 stream of vapor was directed down the center aisle. The generator was operated for 

 20 or 30 minutes on 5 consecutive days. Cages of insects were used in this test also. 



5/ Gillenwater, Hagen B., and Phillip K. Harein. A Dispenser Designed to 

 Provide Large Quantities of Insecticide Vapor. Jour. Econ. Entomol. 57(5): 76? 764. 

 1964. 



