LAMBOTTE, SEPTEMBER 25, 1902. 53 
4, He gives a geographical distribution of foul brood, having deter- 
mined it by the isolation of B. alvei from diseased larve. 
5. He reports the finding of Bacillus alver in the ovaries of queens, 
and concludes that the eggs in diseased hives might sometimes be 
affected. 
6. He has given quite a lengthy description of B. alvev. 
7. He was not able to isolate B. alver from chilled brood as the con- 
dition is found in the apiary. 
8. He performed various experiments to prove the value of drugs 
in the treatment of foul brood, with the conclusion that drugs in 
certain cases—for example, formic acid and _ beta-naphthol—are 
undoubtedly very useful, while some others are of very little or no 
value. 
9. He also reports the successful production of the disease with 
typical symptoms by feeding the spores from pure cultures of B. alvet. 
10. He mentions no difficulty in obtaining B. alver from ‘“foul- 
brood” material. , 
The authors of this bulletin disagree with nearly all of the points 
made by Harrison. His failure to recognize the fact that the mass 
of spores, which are always seen in brood dead of American foul brood, 
do not grow when plate cultures are made, was fatal to his work. 
Occasionally colonies of Bacillus alvei do appear on plates, made from 
this disease, but when they do they appear only in relatively small 
numbers. . 
LAMBOTTE, SEPTEMBER 25, 1902. 
A paper by Lambotte* caused, at the time it was written, con- 
siderable discussion among bee keepers. It caused some comment 
also on the part of others on account of the views which he expressed 
concerning the identity of Bacillus mesentericus vulgaris and Bacillus 
alver, and the relation of Bacillus mesentericus vulgaris to foul brood. 
In view of the fact that foul brood seemed to appear unexpectedly 
away from all known sources of infection, a beekeeping society peti- 
tioned the minister of agriculture of Belgium to have a new scientific 
study of foul brood made. Lambotte’s work is the result of this 
petition. From requests made through journals, an ample supply 
of foul-brood samples was received. The diseased larvee in capped 
cells were recognized by the darkened, sunken, and usually perforated 
-cappings. The brood dead of the disease he describes as being 
yellow or brownish-yellow, viscid, ropy, and emitting a nauseating 
odor. 
From a stained preparation made from ropy larve, Lambotte 
observed a very large number of spores which he supposed were the 
1Lambotte, Dr. Ul., September 25, 1902. Recherches sur le microbe de la “loque,’? maladie des 
abeilles. Travail du laboratoire de l’institut de pathologie et de bacteriologie de l’université de Liége. 
Annales de l'Institut Pasteur, Tome XVI, No. 9, pp. 694-704. 
