1910.] Bacterial Endotoxins on Phagocytosis, ete. ao. 
phagocytosis was unaffected. It is only necessary to cite one experiment, as 
all our results were identical :— 
No. of No. of 
bacilli in | non-phagocytie 
50 cells. cells. 
A. Normal saline + B. coli + normal leucocytes + normal serum...... 361 0 
+ B. Danysz + normal leucocytes + normal serum 313 1 
B. Coli extract + B. coli+ normal leucocytes + normal serum ...... 4.20 | 0 
+ B. Danysz + normal leucocytes + normal serum . 339 0 
C, Danysz extract + B. coli + + vs 449 | (6) 
is + B. Danysz + nor mal leucocytes + normal serum 385 0 
Ts the action of the Endotoxie Substance on the Serum ? 
As it was evident that the action of this body was not directly related to 
the leucocytes or the organisms themselves, further investigations, mostly of a 
confirmatory nature, were undertaken in connection with its action on the 
serum. The same technique has been employed as given in detail in our 
previous report. 
Numerous experiments were made for the purpose of ascertaining whether 
absorption of complement and inhibition of phagocytosis, resulting from the 
action of endotoxin upon normal serum, were directly related. It was found, 
however, that no greater phagocytosis occurred in those instances where 
ample hemolytic complement was still present at the end of the period of 
interaction than if all the complement had been absorbed by the endotoxic 
substance. For the hemolytic experiments either sheep’s red cells or 
sensitised human red cells were employed. The following experiment may 
be cited to illustrate these points :-— 
| 
| No. of pe Hemolytic 
| bacilli in ea caere experi- 
50 cells, | POBECCY4C) ments. 
| celle. | 
A. Serum saline* + leucocytes + B. typhosus .......60...0..00 ee lOU 8 | Complete 
+ _ + B. paratyphotd .....+....+. 118 3 hemolysis. 
B. Normal serum paratyphoid extract + leucocytes 65 16 
+ B. typhosus ... ig No hemo- 
i - 4 + leucocytes lysis. 
+ B. paratyphoid I Lb a 
C. Normal serum typhoid extract vests + B. typhosus 8 43 Nauk aovee 
9 3) ? 3) ‘ 7 
+ B. paratyphoid I ae 7 lysis. 
* By “serum saline,” “serum paratyphoid extract,” etc..is meant one volume of a mixture of 
equal parts of serum and saline, or of serum and the bacterial extract in question, after one hour’s 
incubation at 37° C. 
Daz 
