80 Miss M. P. FitzGerald. The Origin of the [June 4, 
the blue compound. In others, it was observed only in the upper portion of 
the crypts of the glands; on the surface of the gland cells, or in structures 
external to the gland tubules; but in one of the rabbits proof of the most 
decisive character was obtained, both of the presence of the deposit of Prussian 
blue in the central lumina of the gland tubules and of the occurrence of the 
reaction within the parietal cells. 
This rabbit (Rabbit 2, see Table) received eight injections by the sub- 
cutaneous method, during the space of 30 hours. The total amount of the 
solution of the two salts injected was 32 c. cms., and this was given in quan- 
tities varying from 4 to 6 c. cms.; 23 c. cms. were given during the first 
12 hours of the experiment, the five injections being given at intervals of 
14 to 12 hours. After receiving this amount (23 c. ems.) the rabbit was 
allowed to rest for about 17 hours. During the subsequent seven hours 
three injections of 4 to 5 c. cms. of the fluid were given at intervals of 
2 or 24 hours. 
The animal partook heartily of carrots supphed during the experiment. 
No toxic effect due to the injection fluid was observed. The earliest 
evidence of the excretion of the two salts contained in the injection fluid 
was obtained in the urine voided about six hours after the first injection. 
The rabbit was killed 30 hours after the first and 24 hours after the last 
injection had been administered. 
In this instance, only a slight deposit of Prussian blue was found adhering to 
the surface of the gastric mucosa and in the contents directly in contact with it, 
and this deposit, greenish in colour, was situated in a small area close to the 
cesophageal opening, and in the region adjacent, corresponding to the border 
of the lesser curvature. 
In addition to the superficial deposit, the examination of vertical sections, 
with the low power of the microscope, revealed a deposit of Prussian blue in 
some of the crypts, and at isolated points in the upper two-thirds of the 
central lumina of certain of the gland tubules. (See Plate 7, fig. 2.) 
Occasionally the deposit extended continuously through more than a third 
of the gland to the surface, but more frequently it occurred in isolated 
lengths, and was as a rule confined to that third of the tubule adjoining the 
crypt. The deposit was never found in the lower third of the tubule. It is 
thus seen that the reaction occurred in the neck of the gland, in which 
region the parietal cells predominate. By further examination with the high 
power, the crypts appeared to be lined and filled with a material composed of 
wavy blue strands, frequently of considerable thickness. In these, deeply — 
coloured varicosities occurred at irregular intervals, which were regarded as 
being probably agglomerations of the Prussian blue and mucus. | 
