86° Miss M. P. FitzGerald. The Origin of the [June 4, 
washed rapidly in distilled water after their removal from the solution of the 
double citrate and before being placed in the potassium ferrocyanide solution. 
The results obtained by this method, as might be anticipated, were quite of 
an indeterminate nature. In the case of one guinea-pig, a diffuse greenish 
coloration was observed here and there over the gland tubules, and, in another, 
a peculiar brown crystalline precipitate was observed in the parietal cells. ® 
Table giving Results Obtained by Injecting a Solution containing Equal Parts of an Aqueous 
Solution of 2°25-Per-Cent. Ammonium Ferric Citrate and of 1:°5-Per-Cent. Potassium 
Ferrocyanide. 
. A poeaseee blue Ditto, on addition of 
| Vo. of hours reaction—spontaneous. |. 0°5 per cent. HCl. 
Animal Ne an OTS |) Grates Wass Condition HESe, ib = i 
| inje ctions, | injected. | and last injec- j effect. | ie 
: tions. : : ; arious 
Gastric mucosa. | Skin.| Urine. | iissues:® 
: | | 
c.cms. | First. | Last. Surface. |Interior. 
Rabbit Intra- 10—11 35+ 2 Food given. _ _ = = tt a 
(1) venous, 1 
Subcuta- | 
neous, 2 | 
Rabbit Subcuta- 37 30 25 Food given. — ae 4 + 4 
(2) neous, 5 (1st day) 
(2nd day) | 
Rabbit | Subcuta- 31—32 25} 25 Starved 24 hrs. = + — - = | + 
(3) neous, 5 (Ist day) | (Retention 
aP of urine) 
(2nd day) 
Rabbit | Intra- 17 4} 23 | Semi-fasting. (Starved - = _ _ + ok 
(4) venous, 2 36 hrs. Food. 
Starved 24 hrs.) 
Rabbit | Subcuta- 50 65 1 No food eaten during ae + — 
(5) neous, 5 expt. 
Rabbit Subcuta- 43—45 3 3—] Food before exp. _ + — 
(6) neous, 4 
Rabbit Subcuta- 40 55 1 Semi-fasting. Food + _ = — + 
(7) (sod neous, 4 ' given after fast of 20 hrs. 
ferro- ' and during expt. Eaten 
cya- well until after 4th 
nide) injection. 
Guinea- | Subcuta- 22 3f 1 Food given. + _ + + a 
pig (1) neous, 2 
uinea- | Subcuta- 16 5} 3 + - _ +—? + 
pig (2) neous, 2 (Polyuria) 
Guinea- | Subcuta- 19 6} 45 + - + + + 
pig (3) neous, 2 (Polyuria) | 
Dog (1)...! Subcuta- 55 3t 14 Starved 48 hrs. Food + = — — = 2: 
neous, 2 given 1 hr. before expt. | (Vomit- 
ing) 
Dog (2)...| Subeuta- 42 3} + + - + + + 
neous, 2 (Coma- 
tose) 
++ means positive result. 
— means negative result. 
* After their removal from the body, and subsequent exposure to the action of dilute hydrochloric acid, the Prussian blue reaction 
developed, at various times, in the following tissues :—Bladder, kidney, liver, small and large intestines, ovary, fallopian tubes, uterus, 
vagina, and lymph gland of axillary region (dog). The kidney became quite blue in many cases, and under the microscope the 
uriniferous tubules presented a very striking appearance. 
In view of the proof obtained of formed acid existing in the canaliculi of 
the parietal cells, it was a matter both of interest and of importance to repeat 
the experiments of Mary Greenwood* and of Macallum,t referring to the 
* M. Greenwood, zbid. 
+ A. B. Macallum, ‘Ergebn. d. Physiol.” 1908, vol. 7, p. 628. 
