1910. | with the State of Aggregation of Matter. 115 
between the chloride, bromide, and nitrate and iodide are similar to the 
The results of Osborne and Harris 
have been extended by determining the solubility of edestin in a series of 
actions in the case of serum globulin. 
organic salts, and the results are indicated in the accompanying Table V. 
The edestin was prepared from hemp seed in the same way as that 
employed by Osborne and Harris, the final recrystallisation from sodium 
chloride having been carried out, after neutralisation of the solution, by 
allowing the latter to cool in an atmosphere free from carbon dioxide. 
The experiments were carried out at ordinary room temperature in the 
same way as that described in Osborne and Harris’ paper. Suspensions of 
1 gramme in 10 ce. of solution or of 2 cc. in 20 cc. solution were shaken 
for some time. When accurate quantitative determinations were made, the 
mixture was centrifuged, and the nitrogen determined in the supernatant 
liquid, which was pipetted off. 
It was noticed that certain limits of concentration were reached between 
which the solubility of the edestin rapidly increased (compare the curves in 
Osborne and Harris’ paper). At these limits the edestin became gelatinous 
on the addition of the salt solution. For these reasons the great differences 
of the solution capacities of the different salts could be readily demonstrated 
without the necessity of carrying out nitrogen estimations ; in fact, when the 
edestin became gelatinous, accurate determinations of solubility were difficult. 
Table V.—Solubility of Edestin in Sodium Salts of Organic Acids. 
P. indicates that the 
Edestin forms a paste on addition of Salt. 
Numbers indicate grammes dissolved in 10 c.c. 
nee Normal solution. 3N/10. N/2. N/4. N/10 
| Hormiatelseceetcee: Insol. Insol Insol Insol. Insol 
Lactate: Aiics.c.. 5. ty - - i i, | 
Acetate ............ 5 | i eS . » | 
| Monochloracetate Forms paste 6°53 0°87 9 ” 
Dichloracetate ... Very sol. P. Very sol. P.| Very sol. P. 0°45 » | 
Trichloracetate ... ‘e ‘s By 5°56 | Traces only sol. | 
Benzoate ......... 4 * 7 5°07 . | 
Salicylate ......... mn Very sol. Very sol. Very sol. 1°46 | 
Forms gel on 
addition of 3 gm. 
to 20 c.c. | 
Malonate ......... 1 ‘86 1°65 Traces only sol.; Insol Insol | 
Succinate ......... 0°65 0°29 Rs 5 3 | 
Martratess.cscseten 1 94, 0-78 + es 9 
@itrates oss. ecneee 2°48 1°83 S 3 3 
It awa be noticed that the polybasic acid salts have only slightly greater 
solvent capacities for edestin than those of the monobasic acids. 
Furthermore, 
