146 Messrs. H. G. Plimmer and others. [Sept. 19, 
experiments are of interest as showing the influence on the trypanosomes, 
and the constant effects, of one dose of antimony. 
Table giving Details of the above-mentioned Experiments. 
: Death Trypanosomes present 
Rats inoculated from occurred Spleen. (+) or absent (0) Remarks. 
treated Rat 1 on— 
onu— from blood. 
| 
5th day after dose... — — — Living 103 days. 
ies es x ...| 31st day | Very large 0 
Othe es; & ...| 16th day | Large 0 
wth ., i hc — — — Living 95 days. 
14th _,, a ...| 138th day | Very large 0 ; 
Ditto from Rat 2 on—' 
2nd day after dose... ~- — —_ Living 52 days. 
4th __,, 5 — - — — Living 50 days. 
iGhipe & ae — | = _ Living 47 days. 
Oth: = ¥,; 2 ...| 28th day | Very large ++ 
16th — ;, ...| 41st day Enormous 0 in blood, spleen, 
3 liver, adrenals. 
It will be noticed that death was delayed up to as long as 41 days. Two 
other. rats were given a relatively smaller dose of antimony, and rats 
inoculated from these on the 4th, 7th, 9th, and 11th days after the dose died 
with trypanosomes in their blood on the 24th, 19th, 19th, and 29th days 
respectively, the incubation period only being much prolonged. 
Experiments upon Surra Horses with Antimony. 
Inoculated Surra in horses runs a quicker course than that acquired 
naturally, and it is difficult to give a certain time for the disease, as a good 
deal depends on the condition of the horse, and experimental horses are 
usually old and worn-out and would succumb more quickly than young ones. 
Cases of untreated experimental Surra in horses have died as early as the 
14th day, but the time probably depends very much upon the age and 
condition of the animal. The horses we have used were inoculated from rats, 
and the incubation period was practically six days. Musgrave and Clegg* | 
give 6 to 13 days as the incubation period, so that our strain of Surra is a 
fairly virulent one for horses. | 
Both intramuscular and intravenous injection of antimony (metal) have 
been tried. Doses of 2 grammes have been given, intravenously, and if 
sufficiently diluted with warm salt solution no unpleasant symptoms 
* Op. cit. 
