152 ; Messrs. Fowler, Ardern, and Lockett. [Sept. 30, 
It appears, therefore, that the action is accelerated by the addition of 
further nutrient material. 
(c) Cultures on Special Media.—A few cubic centimetres of one of these 
cultures were added toa phenol solution. The disappearance of phenol in 
this case was gradual, commencing in the first day or two—the time taken, 
however, for oxidation was considerably longer than in the case of the broth 
cultures, viz. 16 days. 
Results :— 
Oxygen absorbed from acid 
permanganate in 3 minutes. 
Parts per 100,000. 
June 29. Experiment commenced ......... 20°8* 
oJ Why ool a ee ocak atee ecb a nates se een tte os oe 20°4 
50) Wienssantisblslten onesie sesame «ova emeneenctatt 18°0 
Sh tt iteiswsteocueteins «o's's siete tat letetsoicis ais toa iaereane eine 79 
9) tm a A teictetis osisettne's sa:oietante noeteite se aemionioeianelastees 1°4 
* Equivalent to 13°6 parts of phenol per 100,000. 
- Gelatine plates were made from the solutions and it was found, generally, 
that the numbers of the chromogenic organism present in the solutions, 
being several millions per cubic centimetre at commencement, were consider- 
ably increased during the experiment. | 
Proportionally, the number of organisms other than the chromogenic 
present in the solutions were few. 
(d) Addition of a Culture of the Chromogenic Organism to a Solution 
previously unacted upon by B. liquefaciens fluorescens——An interesting bottle 
experiment was made as follows :—To a solution of phenol previously unacted 
upon by a culture of B. liquefaciens as described in the preceding, a broth 
culture of the chromogenic organism was added. Within four days of the 
addition of the second cuiture almost complete oxidation of the phenol had 
taken place. 
Results :— 
Oxygen absorbed from acid 
permanganate in 3 minutes. © 
Parts per 100,000. 
March 2. Experiment commenced ...... 17°40* 
PATON LED ad ere cn ere ce ae eta <uie'asesisecn oes cas 17:00 
» 12 after addition of second culture 19°20 
RY Be ee a a 18°60 
sy ATEN ten eee ON tie tT. og tn 811 
TO 5 EON ea ee 9°46 
* Equivalent to 11°4 parts of phenol per 100,000. 
Gelatine plates of the solution at end of experiment showed presence of 
large number of both liquefying and non-liquefying organisms. 
