214 Dr. H. B. Fantham. History of T. gambiense [Oct. 15, 
be accurate, and many details, as seen in the living parasite, as well as 
numerical data from daily counts of the trypanosomes, are supplied in 
proof of the significance of the latent bodies. Animals inoculated with 
T. rhodesiense (Stephens and Fantham) have also been carefully investigated. 
The Relation between Latent Bodies and the Number of Trypanosomes in the 
Peripheral Blood, with Notes on the Parasites found in the Internal 
Organs of the Hosts. 
Following on the work of R. Ross and D. Thomson (1910) on the 
enumeration of the parasites in the peripheral blood of a patient suffering 
from Rhodesian Sleeping Sickness, the periodic increase in the number of 
trypanosomes in the peripheral blood of rats and guinea-pigs inoculated with 
T. rhodesiense or with T. gambiense has recently been found by Fantham and 
J. G. Thomson. 
Rats similarly inoculated and exhibiting such periodic increase and 
decrease were killed at various points in the cycle, as set forth in the 
following tables :— 
A. Rat 1—TZ. gambiense, Laboratory Strain, in Piebald ¢ Rat, weight 
150 grammes, inoculated with 50,000 trypanosomes. 
Way. cneay 1 2 | 3 4: 5 | 6 7 8 9 
Parasites, perc. mm.| — — — — — — 4, 1088 4392 
Memap, veer ssce. cee es 55 46 70 62 57 | «54 60 
DBVie coy teens se 10 | WL | 12 13 14; 15 
Parasites, per c. mm. 868 1656 2560 9288 6304 436, killed 
Tempe. eee are | 68 44, 46 57 54 61 
* The temperature is expressed according to the following convention, as in Major Ross’s 
recent papers on “ Malaria” and “ Trypanosomiasis ” :—Temp. = (F—95) x 10, where F is the 
temperature in degrees Fahrenheit, recorded with a clinical thermometer. 
Heart blood of Rat 1 examined fresh, immediately after killing: a few 
living trypanosomes seen and some (fewer) rounded forms. 
Spleen : very large; three to seven rounded forms seen in every field of the 
microscope (2 mm. objective and 2 ocular); no flagellates seen in 
fresh preparation. 
Liver and portal blood: living flagellates and rounded bodies seen in about 
equal numbers (one of each per field). 
