220 Dr. H. B. Fantham. History of T. gambiense [Oct. 15, 
In the formation of rounded, latent bodies, as seen im vitro, a portion of 
the body of the flagellate, after passage of the blepharoplast towards the 
nucleus, is thrown off, and the flagellum is discarded (text-fig. 1). Examina- 
tion of preparations of the internal organs (lungs, heart, spleen) of the host 
shows that a similar method of formation of the post-flagellate stages of the 
parasite usually occurs in the internal organs (figs. 1-5). However, in the 
peripheral blood, after careful searching of a sufficient quantity of blood 
(4 cubic millimetre), a few rounding or rounded parasites can generally be 
seen (figs. 12, 17-20). Also, in the internal organs, stages of the parasite 
intermediate between flagellate and non-flagellate forms may be seen (fig. 33). 
The post-flagellate or latent bodies vary in size (figs. 2-16, 22). This 
variation is due to two causes: (1) the formation of non-flagellate parasites 
from trypanosomes of different breadths, and (2) the occasional division of 
large post-flagellate forms by binary fission, an example of the simplest 
schizogony (fig. 23). This fission, so far as my researches go, is infrequent 
in the case of 7. gambiense and T. rhodesiense. It has been observed that 
division of flagellate trypanosomes may immediately precede the formation of 
latent bodies. 
Broad forms of 7. rhodesiense, with posterior nucleus, may form relatively 
large latent bodies. As the nucleus is at or near the posterior (non- 
flagellar) end of the parasite, there is little of the body discarded in that 
region when rounding occurs. The kidney shape of the nucleus of some 
specimens of 7. rhodesiense is seen in their latent bodies (fig. 27), and the 
nucleus lies to one side of the rounding body (figs. 17, 18). It is not easy, 
however, to differentiate between the latent bodies of TZ. rhodesiense and 
T. gambiense. 
Moore and Breinl describe a stainable band or black line connecting the 
nucleus and blepharoplast of certain specimens of 7. gambiense at or near the 
maxima. An “interaction” takes place between the blepharoplast and 
nucleus. After this the formation of latent bodies proceeds. During the 
researches now recorded, there was no good evidence found in support of 
Moore and Breinl’s views. The stainable band was seen in some stout 
(probably old) parasites,*and in parasites at periods near the death of the 
host. It is possible, as Swellengrebel (1908) suggested, that the stainable 
line is a form of degeneration. However, on the exact significance of the 
stainable band seen in such trypanosomes I prefer not to pronounce a 
definite opinion at present. 
