Treatinent of Ammals mnfected with Trypanosomes. 227 
Fig. 41 represents an almost trypaniform stage of 7. gambiense from blood of guinea-pig. 
Note the peculiar position of the blepharoplast (kinetonucleus). 
Fig. 42 represents a stout trypaniform parasite from heart blood of rat. 
Fig. 43 shows peculiar, large rounding form (from blood of a rat), exhibiting signs of 
division of its nucleus and blepharoplast. 
_ Figs. 44, 45.—Slightly irregular parasites, very pale staining and vacuolated. Degenerating, 
as seen in detachment of flagellum, etc. 
Figs. 46—49.—Degenerating trypanosomes, exhibiting chromatolysis, all from lungs of 
rats. Fig. 46 of 7. gambiense, magnified 1350. | 
Fig. 50.—Degenerating Trypanosoma gambiense, showing vacuolation, pale-staining 
cytoplasm and beginning of disintegration. 
f 
Expervments on the Treatment of Animals Infected with Trypano- 
somes, by means of Atoxyl, Vaccines, Cold, X-rays, and 
Leucocytic Kxtract; Enumerate Methods Employed.  (Pre- 
luminary Note.) 
By Prof. Major R. Ross, C.B., F.R.S8., and J. G. THomson, M.A., M.B., Ch.B. 
(Received October 15,—Read December 8, 1910.) 
These experiments were conducted with funds given by Sir Edwin 
Durning-Lawrence, Bart., for the purpose of testing the effect of cold on 
disease, and were suggested by Major R. Ross as a part of the studies 
made in connection with a case of Sleeping Sickness reported upon in an 
accompanying paper by him and Dr. David Thomson. Although many 
researches have been made on the effect of atoxyl and other drugs, we 
believe that these are the first in which that effect has been measured by 
regular daily counts of the parasites by measured thick film methods. 
ey Atorcyl. 
From a study of the patient W. A. at the Royal Southern Hospital by 
Major R. Ross and D. Thomson, it will be noted that atoxyl failed to be 
of any marked benefit to the patient, and in the doses administered there 
was no noticeable trypanocidal action. Various doses of atoxyl are 
recommended in treatment of human trypanosomiasis, and the patient (W. A.) 
received 4 grains as a maximum dose. It was impossible in this case to 
push the drug further, as the patient quickly showed signs of the toxic 
action of the drug. 
Louis Martin and Henri Darré* believe that atoxyl can cure light forms 
* © Bulletin of Sleeping Sickness,’ 1910, vol. 2, No. 18. 
