232 Major R. Ross and Mr. J. G. Thomson. [ Oct. 15, 
III. Treatment by Means of Cold. 
Here most interesting results have been obtained. Sir Edwin Durning- 
Lawrence, Bart., has taken great interest in the effect of cold in disease, 
especially tropical disease, and owing to his assistance these experiments have 
been made possible. | 
A chamber 12 feet long by 7 feet wide by 6? feet high was constructed, 
and this is cooled by a refrigerator made by Sir Alfred Haslam. The lowest 
temperature reached in our experiments was 20° F. The humidity of the 
atmosphere in this chamber was low and varied between 50 per cent. and 
60 per cent., but this varied with the humidity of the outside atmosphere, 
as the door had to be opened several times daily for the purposes of 
observation. 
Preliminary experiments were carried out in this cold chamber by Major 
Williams, and his results have been published in ‘ Annals of Tropical 
Medicine,’ Liverpool, July, 1910. 
Major Williams compares the atmosphere of the cold chamber to the 
climate of the interior of Canada. The patient, W.A., suffering from Sleeping 
Sickness contracted in Rhodesia, several times visited the cold chamber for 
treatment ; but, unfortunately, at this time no counts were made of the 
parasites in the peripheral blood. We have, however, the evidence of the 
patient himself, who emphatically declared that he felt much better after 
being for some time in the cold. As the patient became worse, treatment had 
to be discontinued, owing to the fact that the patient was considered too ill 
to travel from the Hospital to the University; and we had to resort to 
treatment of subinoculated animals. 
We used two strains of trypanosomes :— 
(1) The Rhodesian strain (7. rhodesiense, Stephens and Fantham),. 
(2) The old laboratory strain of Z’ gambiense. 
The essential difference between these two strains has been discussed in 
an accompanying paper on enumerative methods in untreated animals. by 
H. B. Fantham and J. G. Thomson. 
Five guinea-pigs were kept in the animal house, and four were kept in the 
cold chamber. Of these seven were subinoculated with the old laboratory 
strain of 7. gambiense, and two were inoculated with the Rhodesian strain. 
The average incubation period of the controls in the animal house was 
four days, whereas the average incubation period of those treated in the cold 
was 13°5 days. 
The average duration of life of the controls was 64:2 days, whereas the 
average life of those in the cold was 97°25 days. In addition to this the 
