242 Dr. W. Yorke. Auto-Agglutination of [ Nov. 2, 
Lso-agglutination—A._ series of experiments were undertaken with a view 
to ascertaining whether the sera of those infected animals which possessed 
‘the property of agglutinating their own red cells were also capable of 
producing agglutination of the erythrocytes of other animals of the same 
species. In every case where auto-agglutination was present, iso-agglutination 
was found to occur when the serum of the infected animal was added to 
the red cells of ancther animal (either normal or infected) of the same species. 
Analogous results were obtained with the blood of a case of human 
trypanosomiasis in Major Ross’s clinic in Liverpool. Cover-slip preparations 
of the blood of this case exhibited a certain degree of auto-agglutination 
during the six months he was under observation. The following table gives 
the results of an examination of his blood, drawn 10 days before his death, for 
auto- and iso-agglutinins. 
Table I—Examination of Sleeping Sickness Serum for Auto- and 
Iso-agglutinin. 
Equal volumes of serum and red blood cell 
suspension used. 
5 per cent. suspension | eT Aa ee PH Alene: JeeSIetEl 
of washed erythrocytes | Le Ra 
in normal saline eer 
solution. set prea a 
aC. 
Sleeping Sickness......... Sleeping Sickness....., 37 No agglutination in 30 mins. 
18 Marked 45 30 —, 
0 Complete __,, LOMA 
Normal individual A ...; Sleeping Sickness...... 37 No agglutination in 30 _,, 
18 Complete __,, 30). 4; 
0 9) 29 10 99 
Normal individual B ...! Sleeping Sickness...... 37 No agglutination in 30 ,, 
18 Marked 5) 30 —,, 
0 Complete _,, LOw 4s 
‘| Normal individual C ...| Sleeping Sickness...... 37 No agglutination in 30 __,, 
ih 18 Marked fe 30 C=» 
0 Complete __,, LO" 
Normal individual C ...| Normal individual C 37 No agglutination in 30 _,, 
| 18 3) 3) 30 ” 
0 ” ” 30 ,, 
Normal individual C .... Normal individual B | 387 No agglutination in 30 _,, 
| 18 %» » 30 5, 
0 Marked % 30g 
Spontaneous Agglutination of the Red Cell Suspensions.—On rare occasions it 
was found that the 5-per-cent. suspension of red cells which had been washed 
